7jta

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<StructureSection load='7jta' size='340' side='right'caption='[[7jta]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='7jta' size='340' side='right'caption='[[7jta]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7jta]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JTA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7jta]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridia_bacterium Clostridia bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JTA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.801&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jta OCA], [https://pdbe.org/7jta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jta RCSB], [https://www.ebi.ac.uk/pdbsum/7jta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jta ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jta OCA], [https://pdbe.org/7jta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jta RCSB], [https://www.ebi.ac.uk/pdbsum/7jta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jta ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A5B9TEE9_9BACT A0A5B9TEE9_9BACT]
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To overcome CRISPR-Cas defense systems, many phages and mobile genetic elements (MGEs) encode CRISPR-Cas inhibitors called anti-CRISPRs (Acrs). Nearly all characterized Acrs directly bind Cas proteins to inactivate CRISPR immunity. Here, using functional metagenomic selection, we describe AcrIIA22, an unconventional Acr found in hypervariable genomic regions of clostridial bacteria and their prophages from human gut microbiomes. AcrIIA22 does not bind strongly to SpyCas9 but nonetheless potently inhibits its activity against plasmids. To gain insight into its mechanism, we obtained an X-ray crystal structure of AcrIIA22, which revealed homology to PC4-like nucleic acid-binding proteins. Based on mutational analyses and functional assays, we deduced that acrIIA22 encodes a DNA nickase that relieves torsional stress in supercoiled plasmids. This may render them less susceptible to SpyCas9, which uses free energy from negative supercoils to form stable R-loops. Modifying DNA topology may provide an additional route to CRISPR-Cas resistance in phages and MGEs.
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The novel anti-CRISPR AcrIIA22 relieves DNA torsion in target plasmids and impairs SpyCas9 activity.,Forsberg KJ, Schmidtke DT, Werther R, Uribe RV, Hausman D, Sommer MOA, Stoddard BL, Kaiser BK, Malik HS PLoS Biol. 2021 Oct 13;19(10):e3001428. doi: 10.1371/journal.pbio.3001428., eCollection 2021 Oct. PMID:34644300<ref>PMID:34644300</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7jta" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Clostridia bacterium]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Forsberg, K J]]
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[[Category: Forsberg KJ]]
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[[Category: Stoddard, B L]]
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[[Category: Stoddard BL]]
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[[Category: Werther, R]]
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[[Category: Werther R]]
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[[Category: Anti-crispr]]
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[[Category: Cas9]]
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[[Category: Nuclease]]
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[[Category: Phage protein]]
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[[Category: Unknown function]]
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Revision as of 07:16, 3 April 2024

Crystal structure of a putative nuclease with anti-Cas9 activity from an uncultured Clostridia bacterium

PDB ID 7jta

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