1tzn

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(New page: 200px<br /> <applet load="1tzn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tzn, resolution 4.30&Aring;" /> '''Crystal Structure o...)
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Revision as of 17:23, 12 November 2007


1tzn, resolution 4.30Å

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Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore bound to the VWA domain of CMG2, an anthrax toxin receptor

Contents

Overview

After binding to cellular receptors and proteolytic activation, the, protective antigen component of anthrax toxin forms a heptameric prepore., The prepore later undergoes pH-dependent conversion to a pore, mediating, translocation of the edema and lethal factors to the cytosol. We describe, structures of the prepore (3.6 A) and a prepore:receptor complex (4.3 A), that reveal the location of pore-forming loops and an unexpected, interaction of the receptor with the pore-forming domain. Lower pH is, required for prepore-to-pore conversion in the presence of the receptor, indicating that this interaction regulates pH-dependent pore formation. We, present an example of a receptor negatively regulating pH-dependent, membrane insertion.

Disease

Known diseases associated with this structure: Fibromatosis, juvenile hyaline OMIM:[608041], Hyalinosis, infantile systemic OMIM:[608041]

About this Structure

1TZN is a Protein complex structure of sequences from Bacillus anthracis and Homo sapiens with CA and MG as ligands. Full crystallographic information is available from OCA.

Reference

Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation., Lacy DB, Wigelsworth DJ, Melnyk RA, Harrison SC, Collier RJ, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13147-51. Epub 2004 Aug 23. PMID:15326297

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