1jv5

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<StructureSection load='1jv5' size='340' side='right'caption='[[1jv5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='1jv5' size='340' side='right'caption='[[1jv5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1jv5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JV5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1jv5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JV5 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jv5 OCA], [https://pdbe.org/1jv5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jv5 RCSB], [https://www.ebi.ac.uk/pdbsum/1jv5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jv5 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jv5 OCA], [https://pdbe.org/1jv5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jv5 RCSB], [https://www.ebi.ac.uk/pdbsum/1jv5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jv5 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KV133_HUMAN KV133_HUMAN] V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).<ref>PMID:17576170</ref> <ref>PMID:20176268</ref> <ref>PMID:22158414</ref> <ref>PMID:24600447</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jv5 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jv5 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
 
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== Publication Abstract from PubMed ==
 
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The specificity of antibody recognition of the ABO blood group trisaccharide antigens has been explored by crystal structure analysis and mutation methods. The crystal structure of the Fv corresponding to the anti-blood group A antibody AC1001 has been determined to 2.2-A resolution and reveals a binding pocket that is complementary to the blood group A-trisaccharide antigen. The effect of mutating specific residues lining this pocket on binding to the A and B blood group oligosaccharide antigens was investigated through a panel of single point mutations and through a phage library of mutations in complementarity determining region H3. Both approaches gave several mutants with improved affinity for antigen. Surface plasmon resonance indicated up to 8-fold enhancement in affinity for the A-pentasaccharide with no observable binding to the blood group B antigen. This is the first example of single point mutations in a carbohydrate-binding antibody resulting in significant increases in binding affinity without loss of specificity.
 
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Structure of an anti-blood group A Fv and improvement of its binding affinity without loss of specificity.,Thomas R, Patenaude SI, MacKenzie CR, To R, Hirama T, Young NM, Evans SV J Biol Chem. 2002 Jan 18;277(3):2059-64. Epub 2001 Oct 25. PMID:11679577<ref>PMID:11679577</ref>
 
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
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</div>
 
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<div class="pdbe-citations 1jv5" style="background-color:#fffaf0;"></div>
 
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Evans, S V]]
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[[Category: Evans SV]]
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[[Category: Hirama, T]]
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[[Category: Hirama T]]
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[[Category: MacKenzie, C R]]
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[[Category: MacKenzie CR]]
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[[Category: Patenaude, S I]]
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[[Category: Patenaude SI]]
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[[Category: Thomas, R]]
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[[Category: Thomas R]]
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[[Category: To, R]]
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[[Category: To R]]
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[[Category: Young, N M]]
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[[Category: Young NM]]
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[[Category: Immune system]]
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[[Category: Immunoglobulin]]
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[[Category: Sugar binding protein]]
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Revision as of 07:55, 3 April 2024

Anti-blood group A Fv

PDB ID 1jv5

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