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1rio

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[[Image:1rio.gif|left|200px]]
[[Image:1rio.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1rio |SIZE=350|CAPTION= <scene name='initialview01'>1rio</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1rio", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= CI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])
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-->
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|DOMAIN=
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{{STRUCTURE_1rio| PDB=1rio | SCENE= }}
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|RELATEDENTRY=[[1ku7|1KU7]], [[1lmb|1LMB]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rio OCA], [http://www.ebi.ac.uk/pdbsum/1rio PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rio RCSB]</span>
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}}
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'''Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA'''
'''Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA'''
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[[Category: Nickels, B E.]]
[[Category: Nickels, B E.]]
[[Category: Sun, L.]]
[[Category: Sun, L.]]
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[[Category: helix-turn-helix]]
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[[Category: Helix-turn-helix]]
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[[Category: transcription activation]]
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[[Category: Transcription activation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:32:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:28:40 2008''
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Revision as of 04:32, 3 May 2008

Image:1rio.gif

Template:STRUCTURE 1rio

Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA


Overview

The cI protein of bacteriophage lambda (lambdacI) activates transcription by binding a DNA operator just upstream of the promoter and interacting with the RNA polymerase sigma subunit domain 4 (sigma(4)). We determined the crystal structure of the lambdacI/sigma(4)/DNA ternary complex at 2.3 A resolution. There are no conformational changes in either protein, which interact through an extremely small interface involving at most 6 amino acid residues. The interactions of the two proteins stabilize the binding of each protein to the DNA. The results provide insight into how activators can operate through a simple cooperative binding mechanism but affect different steps of the transcription initiation process.

About this Structure

1RIO is a Protein complex structure of sequences from Enterobacteria phage lambda and Thermus aquaticus. Full crystallographic information is available from OCA.

Reference

Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:14731393 Page seeded by OCA on Sat May 3 07:32:44 2008

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OCA

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