1lk3

<table><tr><td colspan='2'>[[1lk3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LK3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1LK3 FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1j7v|1j7v]]</div></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IL10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1lk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lk3 OCA], [http://pdbe.org/1lk3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lk3 RCSB], [http://www.ebi.ac.uk/pdbsum/1lk3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lk3 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/IL10_HUMAN IL10_HUMAN]] Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

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== Publication Abstract from PubMed ==

IL-10 is a dimeric cytokine that must engage its high-affinity cell surface receptor, IL-10R1, to induce multiple cellular activities. Here we report the 1.9 A crystal structure of an engineered IL-10 monomer (IL-10M1) in complex with a neutralizing Fab fragment (9D7Fab). 9D7Fab and IL-10R1 bind distinct nonoverlapping surfaces on IL-10M1. Antagonism of the IL-10M1/IL-10R1 interaction is the result of 9D7Fab-induced conformational changes in the CD loop of IL-10M1 that indirectly alter the structure of the IL-10R1 binding site. A single mutation (Ile87Ala) in the same CD loop region of the Epstein-Barr virus IL-10 (ebvIL-10) also reduces IL-10R1 binding affinity, suggesting that ebvIL-10 and 9D7Fab use similar allosteric mechanisms to modulate IL-10R1 affinity and biological activity.

Noncompetitive antibody neutralization of IL-10 revealed by protein engineering and x-ray crystallography.,Josephson K, Jones BC, Walter LJ, DiGiacomo R, Indelicato SR, Walter MR Structure. 2002 Jul;10(7):981-7. PMID:12121653<ref>PMID:12121653</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1lk3" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Human]]

[[Category: DiGiacomo, R]]

[[Category: Indelicato, S R]]

[[Category: Jones, B C]]

[[Category: Josephson, K]]

[[Category: Walter, L J]]

[[Category: Walter, M R]]

[[Category: Immune system]]