This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1rjx
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1rjx.jpg|left|200px]] | [[Image:1rjx.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1rjx", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1rjx| PDB=1rjx | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT''' | '''Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT''' | ||
| Line 34: | Line 31: | ||
[[Category: Zhai, P.]] | [[Category: Zhai, P.]] | ||
[[Category: Zhang, X C.]] | [[Category: Zhang, X C.]] | ||
| - | [[Category: | + | [[Category: Microplasminogen]] |
| - | [[Category: | + | [[Category: Plasminogen activation]] |
| - | [[Category: | + | [[Category: Streptokinase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:35:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:35, 3 May 2008
Human PLASMINOGEN CATALYTIC DOMAIN, K698M MUTANT
Contents |
Overview
Streptokinase (SK) is a human plasminogen (Pg) activator secreted by streptococci. The activation mechanism of SK differs from that of physiological Pg activators in that SK is not a protease and cannot proteolytically activate Pg. Instead, it forms a tight complex with Pg that proteolytically activates other Pg molecules. The residue Lys-698 of human Pg was hypothesized to participate in triggering activation in the SK-Pg complex. Here, we report a study of the Lys-698 to Met substitution in the catalytic domain of Pg (microPg) containing the proteolytic activation-resistant background (R561A). While it remains competent in forming a complex with SK, maintaining a comparable equilibration dissociation constant (K(D)), the recombinant protein shows a nearly 60-fold reduction in amidolytic activity relative to its R561A background when mixed with native SK. A 2.3 A crystal structure of this mutant microPg confirmed the correct folding of this recombinant protein. Combined with other biochemical data, these results support the premise that Lys-698 of human Pg plays a functional role in the so-called N-terminal insertion activation mechanism by SK.
Disease
Known disease associated with this structure: Conjunctivitis, ligneous OMIM:[173350], Plasminogen Tochigi disease OMIM:[173350], Plasminogen deficiency, types I and II OMIM:[173350], Thrombophilia, dysplasminogenemic OMIM:[173350]
About this Structure
1RJX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Characterization of Lys-698-to-Met substitution in human plasminogen catalytic domain., Terzyan S, Wakeham N, Zhai P, Rodgers K, Zhang XC, Proteins. 2004 Aug 1;56(2):277-84. PMID:15211511 Page seeded by OCA on Sat May 3 07:35:18 2008
