1m8n
From Proteopedia
(Difference between revisions)
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<StructureSection load='1m8n' size='340' side='right'caption='[[1m8n]], [[Resolution|resolution]] 2.45Å' scene=''> | <StructureSection load='1m8n' size='340' side='right'caption='[[1m8n]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1m8n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1m8n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8N FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8n OCA], [https://pdbe.org/1m8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8n RCSB], [https://www.ebi.ac.uk/pdbsum/1m8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8n ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8n OCA], [https://pdbe.org/1m8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8n RCSB], [https://www.ebi.ac.uk/pdbsum/1m8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8n ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9GSA6_CHOFU Q9GSA6_CHOFU] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8n ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8n ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The insect spruce budworm (Choristoneura fumiferana)(Cf) produces a number of isoforms of its highly active antifreeze protein (CfAFP). Although most of the CfAFP isoforms are in the 9-kDa range, isoforms containing a 30- or 31-amino acid insertion have also been identified. Here we describe the functional and structural analysis of a selected long isoform, CfAFP-501. X-ray crystal structure determination reveals that the 31-amino acid insertion found in CfAFP-501 forms two additional loops within its highly regular beta-helical structure. This effectively extends the area of the two-dimensional Thr array and ice-binding surface of the protein. The larger isoform has 3 times the thermal hysteresis activity of the 9-kDa CfAFP-337. As well, a deletion of the 31-amino acid insertion within CfAFP-501 to form CfAFP-501-Delta-2-loop, results in a protein with reduced activity similar to the shorter CfAFP isoforms. Thus, the enhanced antifreeze activity of CfAFP-501 is directly correlated to the length of its beta-helical structure and hence the size of its ice-binding face. | ||
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| - | A beta-helical antifreeze protein isoform with increased activity. Structural and functional insights.,Leinala EK, Davies PL, Doucet D, Tyshenko MG, Walker VK, Jia Z J Biol Chem. 2002 Sep 6;277(36):33349-52. Epub 2002 Jun 24. PMID:12105229<ref>PMID:12105229</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1m8n" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]] | *[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Choristoneura fumiferana]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Davies | + | [[Category: Davies PL]] |
| - | [[Category: Jia | + | [[Category: Jia Z]] |
| - | [[Category: Leinala | + | [[Category: Leinala EK]] |
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Current revision
Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501
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