1rl3
From Proteopedia
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[[Image:1rl3.gif|left|200px]] | [[Image:1rl3.gif|left|200px]] | ||
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'''Crystal structure of cAMP-free R1a subunit of PKA''' | '''Crystal structure of cAMP-free R1a subunit of PKA''' | ||
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[[Category: Wu, J.]] | [[Category: Wu, J.]] | ||
[[Category: Xuong, N H.]] | [[Category: Xuong, N H.]] | ||
| - | [[Category: | + | [[Category: Camp-dependent protein kinase]] |
| - | [[Category: | + | [[Category: Camp-free]] |
| - | [[Category: | + | [[Category: Crystal structure]] |
| - | [[Category: | + | [[Category: Type 1a regulatory subunit]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:37:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:37, 3 May 2008
Crystal structure of cAMP-free R1a subunit of PKA
Overview
In eukaryotes the primary target for cAMP, a ubiquitous second messenger, is cAMP-dependent protein kinase (PKA). Understanding how binding and release of cAMP changes the cAMP binding domains and then triggers long-range allosteric responses is an important challenge. This conformational switching requires structure solutions of cAMP binding domains in cAMP-bound and cAMP-free states. We describe for the first time a crystal structure of the cAMP binding domains of PKA type Ialpha regulatory subunit where site A is occupied by cGMP and site B is unoccupied. The structure reveals that the carboxyl terminus of domain B serves as a hydrophobic cap, locking the cyclic nucleotide via its adenine ring into the beta-barrel. In the absence of cAMP, the "cap" is released via an extension of the C-terminal helix. This simple hinge mechanism for binding and release of cAMP also provides a mechanism for allosteric communication between sites A and B.
About this Structure
1RL3 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
RIalpha subunit of PKA: a cAMP-free structure reveals a hydrophobic capping mechanism for docking cAMP into site B., Wu J, Brown S, Xuong NH, Taylor SS, Structure. 2004 Jun;12(6):1057-65. PMID:15274925 Page seeded by OCA on Sat May 3 07:37:44 2008
