1oln
From Proteopedia
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==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA== | ==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA== | ||
| - | <StructureSection load='1oln' size='340' side='right'caption='[[1oln | + | <StructureSection load='1oln' size='340' side='right'caption='[[1oln]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br> | <table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , Theoretical Model</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr> | |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA] This protein binds directly to 23S ribosomal RNA. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Thiostrepton and micrococcin inhibit protein synthesis by binding to the L11 binding domain (L11BD) of 23S ribosomal RNA. The two compounds are structurally related, yet they produce different effects on ribosomal RNA in footprinting experiments and on elongation factor-G (EF-G)-dependent GTP hydrolysis. Using NMR and an assay based on A1067 methylation by thiostrepton-resistance methyltransferase, we show that the related thiazoles, nosiheptide and siomycin, also bind to this region. The effect of all four antibiotics on EF-G-dependent GTP hydrolysis and EF-G-GDP-ribosome complex formation was studied. Our NMR and biochemical data demonstrate that thiostrepton, nosiheptide, and siomycin share a common profile, which differs from that of micrococcin. We have generated a three-dimensional (3D) model for the interaction of thiostrepton with L11BD RNA. The model rationalizes the differences between micrococcin and the thiostrepton-like antibiotics interacting with L11BD. | ||
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| - | Structural basis for contrasting activities of ribosome binding thiazole antibiotics.,Lentzen G, Klinck R, Matassova N, Aboul-ela F, Murchie AI Chem Biol. 2003 Aug;10(8):769-78. PMID:12954336<ref>PMID:12954336</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Ribosomal protein L11|Ribosomal protein L11]] | + | *[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]] |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
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[[Category: Streptomyces azureus]] | [[Category: Streptomyces azureus]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
| - | [[Category: Aboul-Ela | + | [[Category: Aboul-Ela F]] |
| - | [[Category: Klinck | + | [[Category: Klinck R]] |
| - | [[Category: Lentzen | + | [[Category: Lentzen G]] |
| - | [[Category: Matassova | + | [[Category: Matassova N]] |
| - | [[Category: Murchie | + | [[Category: Murchie AIH]] |
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Revision as of 05:48, 17 April 2024
Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA
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