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1u6g
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(New page: 200px<br /> <applet load="1u6g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u6g, resolution 3.1Å" /> '''Crystal Structure of...)
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Revision as of 17:25, 12 November 2007
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Crystal Structure of The Cand1-Cul1-Roc1 Complex
Overview
The SCF ubiquitin ligase complex regulates diverse cellular functions by, ubiquitinating numerous protein substrates. Cand1, a 120 kDa HEAT repeat, protein, forms a tight complex with the Cul1-Roc1 SCF catalytic core, inhibiting the assembly of the multisubunit E3 complex. The crystal, structure of the Cand1-Cul1-Roc1 complex shows that Cand1 adopts a highly, sinuous superhelical structure, clamping around the elongated SCF scaffold, protein Cul1. At one end, a Cand1 beta hairpin protrusion partially, occupies the adaptor binding site on Cul1, inhibiting its interactions, with the Skp1 adaptor and the substrate-recruiting F box protein subunits., At the other end, two Cand1 HEAT repeats pack against a conserved Cul1, surface cleft and bury a Cul1 lysine residue, whose modification by the, ubiquitin-like protein, Nedd8, is able to block Cand1-Cul1 association., Together with biochemical evidence, these structural results elucidate the, mechanisms by which Cand1 and Nedd8 regulate the assembly-disassembly, cycles of SCF and other cullin-dependent E3 complexes.
About this Structure
1U6G is a Protein complex structure of sequences from Homo sapiens with ZN as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the Cand1-Cul1-Roc1 complex reveals regulatory mechanisms for the assembly of the multisubunit cullin-dependent ubiquitin ligases., Goldenberg SJ, Cascio TC, Shumway SD, Garbutt KC, Liu J, Xiong Y, Zheng N, Cell. 2004 Nov 12;119(4):517-28. PMID:15537541
Page seeded by OCA on Mon Nov 12 19:32:09 2007
