1ro5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1ro5.gif|left|200px]]
[[Image:1ro5.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1ro5 |SIZE=350|CAPTION= <scene name='initialview01'>1ro5</scene>, resolution 2.30&Aring;
+
The line below this paragraph, containing "STRUCTURE_1ro5", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= LASI, PA1432 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1ro5| PDB=1ro5 | SCENE= }}
-
|RELATEDENTRY=[[1k4j|1k4j]], [[1kzf|1kzf]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ro5 OCA], [http://www.ebi.ac.uk/pdbsum/1ro5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ro5 RCSB]</span>
+
-
}}
+
'''Crystal Structure of the AHL Synthase LasI'''
'''Crystal Structure of the AHL Synthase LasI'''
Line 28: Line 25:
[[Category: Gould, T A.]]
[[Category: Gould, T A.]]
[[Category: Schweizer, H P.]]
[[Category: Schweizer, H P.]]
-
[[Category: alpha-beta-alpha sandwich]]
+
[[Category: Alpha-beta-alpha sandwich]]
-
[[Category: phosphopantetheine fold]]
+
[[Category: Phosphopantetheine fold]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:43:10 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:30:44 2008''
+

Revision as of 04:43, 3 May 2008

Image:1ro5.gif

Template:STRUCTURE 1ro5

Crystal Structure of the AHL Synthase LasI


Overview

The LasI/LasR quorum-sensing system plays a pivotal role in virulence gene regulation of the opportunistic human pathogen, Pseudomonas aeruginosa. Here we report the crystal structure of the acyl-homoserine lactone (AHL) synthase LasI that produces 3-oxo-C12-AHL from the substrates 3-oxo-C12-acyl-carrier protein (acyl-ACP) and S-adenosyl-L-methionine. The LasI six-stranded beta sheet platform, buttressed by three alpha helices, forms a V-shaped substrate-binding cleft that leads to a tunnel passing through the enzyme that can accommodate the acyl-chain of acyl-ACP. This tunnel places no apparent restriction on acyl-chain length, in contrast to a restrictive hydrophobic pocket seen in the AHL-synthase EsaI. Interactions of essential conserved N-terminal residues, Arg23, Phe27 and Trp33, suggest that the N-terminus forms an enclosed substrate-binding pocket for S-adenosyl-L-methionine. Analysis of AHL-synthase surface residues identified a binding site for acyl-ACP, a role that was supported by in vivo reporter assay analysis of the mutated residues, including Arg154 and Lys150. This structure and the novel explanation of AHL-synthase acyl-chain-length selectivity promise to guide the design of Pseudomonas aeruginosa-specific quorum-sensing inhibitors as antibacterial agents.

About this Structure

1RO5 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI., Gould TA, Schweizer HP, Churchill ME, Mol Microbiol. 2004 Aug;53(4):1135-46. PMID:15306017 Page seeded by OCA on Sat May 3 07:43:10 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ro5"
Personal tools