1rpx

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[[Image:1rpx.gif|left|200px]]
[[Image:1rpx.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1rpx |SIZE=350|CAPTION= <scene name='initialview01'>1rpx</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1rpx", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribulose-phosphate_3-epimerase Ribulose-phosphate 3-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.1 5.1.3.1] </span>
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or leave the SCENE parameter empty for the default display.
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|DOMAIN=
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{{STRUCTURE_1rpx| PDB=1rpx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rpx OCA], [http://www.ebi.ac.uk/pdbsum/1rpx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rpx RCSB]</span>
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}}
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'''D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM SOLANUM TUBEROSUM CHLOROPLASTS'''
'''D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM SOLANUM TUBEROSUM CHLOROPLASTS'''
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[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
[[Category: 3-epimerase]]
[[Category: 3-epimerase]]
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[[Category: calvin cycle]]
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[[Category: Calvin cycle]]
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[[Category: chloroplast]]
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[[Category: Chloroplast]]
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[[Category: oxidative pentose phosphate pathway]]
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[[Category: Oxidative pentose phosphate pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:46:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:31:22 2008''
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Revision as of 04:46, 3 May 2008

Image:1rpx.gif

Template:STRUCTURE 1rpx

D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM SOLANUM TUBEROSUM CHLOROPLASTS


Overview

Ribulose-5-phosphate 3-epimerase (EC 5.1.3.1) catalyzes the interconversion of ribulose-5-phosphate and xylulose-5-phosphate in the Calvin cycle and in the oxidative pentose phosphate pathway. The enzyme from potato chloroplasts was expressed in Escherichia coli, isolated and crystallized. The crystal structure was elucidated by multiple isomorphous replacement and refined at 2.3 A resolution. The enzyme is a homohexamer with D3 symmetry. The subunit chain fold is a (beta alpha)8-barrel. A sequence comparison with homologous epimerases outlined the active center and indicated that all members of this family are likely to share the same catalytic mechanism. The substrate could be modeled by putting its phosphate onto the observed sulfate position and its epimerized C3 atom between two carboxylates that participate in an extensive hydrogen bonding system. A mutation confirmed the crucial role of one of these carboxylates. The geometry together with the conservation pattern suggests that the negative charge of the putative cis-ene-diolate intermediate is stabilized by the transient induced dipoles of a methionine sulfur "cushion", which is proton-free and therefore prevents isomerization instead of epimerization.

About this Structure

1RPX is a Single protein structure of sequence from Solanum tuberosum. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of the amphibolic enzyme D-ribulose-5-phosphate 3-epimerase from potato chloroplasts., Kopp J, Kopriva S, Suss KH, Schulz GE, J Mol Biol. 1999 Apr 9;287(4):761-71. PMID:10191144 Page seeded by OCA on Sat May 3 07:46:20 2008

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