1ubi
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(New page: 200px<br /> <applet load="1ubi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ubi, resolution 1.8Å" /> '''SYNTHETIC STRUCTURAL...)
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Revision as of 17:26, 12 November 2007
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SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1
Overview
The small protein ubiquitin (76 amino acids) has been synthesized under, optimized conditions by Merrifield solid-phase methodology using the N, alpha-Fmoc protecting group. The crude polypeptide mixture was purified to, homogeneity by gel filtration, dialysis and a combination of cation- and, anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used, to authenticate the primary structure. Isoelectric focusing and m.s. were, used to demonstrate that the final product was greater than 98% pure with, a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol, scale.
About this Structure
1UBI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin., Ramage R, Green J, Muir TW, Ogunjobi OM, Love S, Shaw K, Biochem J. 1994 Apr 1;299 ( Pt 1):151-8. PMID:8166633
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