2cg4
From Proteopedia
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<StructureSection load='2cg4' size='340' side='right'caption='[[2cg4]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='2cg4' size='340' side='right'caption='[[2cg4]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2cg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2cg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CG4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cg4 OCA], [https://pdbe.org/2cg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cg4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cg4 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cg4 OCA], [https://pdbe.org/2cg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cg4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cg4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/ASNC_ECOLI ASNC_ECOLI] Activator of asnA transcription; autogenous regulator of its own transcription; and repressor of the expression of gidA at a post-transcriptional level.<ref>PMID:3909107</ref> <ref>PMID:2864330</ref> <ref>PMID:2836709</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cg4 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cg4 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The Lrp/AsnC family of transcriptional regulatory proteins is found in both archaea and bacteria. Members of the family influence cellular metabolism in both a global (Lrp) and specific (AsnC) manner, often in response to exogenous amino acid effectors. In the present study we have determined both the first bacterial and the highest resolution structures for members of the family. Escherichia coli AsnC is a specific gene regulator whose activity is triggered by asparagine binding. Bacillus subtilis LrpC is a global regulator involved in chromosome condensation. Our AsnC-asparagine structure is the first for a regulator-effector complex and is revealed as an octameric disc. Key ligand recognition residues are identified together with a route for ligand access. The LrpC structure reveals a stable octamer supportive of a topological role in dynamic DNA packaging. The structures yield significant clues to the functionality of Lrp/AsnC-type regulators with respect to ligand binding and oligomerization states as well as to their role in specific and global DNA regulation. | ||
| - | + | ==See Also== | |
| - | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] | |
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== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Escherichia coli]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Akerboom | + | [[Category: Akerboom J]] |
| - | [[Category: Alonso | + | [[Category: Alonso JC]] |
| - | [[Category: Ayora | + | [[Category: Ayora S]] |
| - | [[Category: Brinkman | + | [[Category: Brinkman AB]] |
| - | [[Category: Muranova | + | [[Category: Muranova T]] |
| - | + | [[Category: Rafferty JB]] | |
| - | [[Category: Rafferty | + | [[Category: Sedelnikova SE]] |
| - | [[Category: Sedelnikova | + | [[Category: Thaw P]] |
| - | [[Category: Thaw | + | [[Category: Wiese S]] |
| - | [[Category: Wiese | + | [[Category: Van der Oost J]] |
| - | [[Category: | + | |
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Current revision
Structure of E.coli AsnC
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