1rsm

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[[Image:1rsm.gif|left|200px]]
[[Image:1rsm.gif|left|200px]]
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{{Structure
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|PDB= 1rsm |SIZE=350|CAPTION= <scene name='initialview01'>1rsm</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1rsm", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=NIN:DINITROPHENYLENE'>NIN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
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{{STRUCTURE_1rsm| PDB=1rsm | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rsm OCA], [http://www.ebi.ac.uk/pdbsum/1rsm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rsm RCSB]</span>
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'''THE 2-ANGSTROMS RESOLUTION STRUCTURE OF A THERMOSTABLE RIBONUCLEASE A CHEMICALLY CROSS-LINKED BETWEEN LYSINE RESIDUES 7 AND 41'''
'''THE 2-ANGSTROMS RESOLUTION STRUCTURE OF A THERMOSTABLE RIBONUCLEASE A CHEMICALLY CROSS-LINKED BETWEEN LYSINE RESIDUES 7 AND 41'''
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[[Category: Sheriff, S.]]
[[Category: Sheriff, S.]]
[[Category: Weber, P C.]]
[[Category: Weber, P C.]]
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[[Category: hydrolase (nucleic acid,rna)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:51:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:32:22 2008''
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Revision as of 04:51, 3 May 2008

Image:1rsm.gif

Template:STRUCTURE 1rsm

THE 2-ANGSTROMS RESOLUTION STRUCTURE OF A THERMOSTABLE RIBONUCLEASE A CHEMICALLY CROSS-LINKED BETWEEN LYSINE RESIDUES 7 AND 41


Overview

The crystal structure of Lys-7-(dinitrophenylene)-Lys-41-cross-linked ribonuclease A has been determined by molecular replacement and refined by restrained least-squares methods to an R factor of 0.18 at 2.0-A resolution. Diffraction intensity data were collected by using a conventional diffractometer and an x-ray area detector. Comparison of the thermostable cross-linked protein and the native enzyme shows them to be structurally similar, with a rms difference in backbone and side-chain atoms of 0.52 and 1.34 A, respectively. Native and modified proteins additionally show 35 common bound solvent sites and similar overall temperature factor behavior, despite localized differences resulting from cross-link introduction, altered crystal pH, or lattice interactions with neighboring molecules. These results are discussed in the context of proposals on the origins of thermostability in the cross-linked enzyme.

About this Structure

1RSM is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The 2-A resolution structure of a thermostable ribonuclease A chemically cross-linked between lysine residues 7 and 41., Weber PC, Sheriff S, Ohlendorf DH, Finzel BC, Salemme FR, Proc Natl Acad Sci U S A. 1985 Dec;82(24):8473-7. PMID:3936036 Page seeded by OCA on Sat May 3 07:51:32 2008

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