2k06
From Proteopedia
(Difference between revisions)
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==Solution structure of the aminoterminal domain of E. coli NusG== | ==Solution structure of the aminoterminal domain of E. coli NusG== | ||
| - | <StructureSection load='2k06' size='340' side='right'caption='[[2k06 | + | <StructureSection load='2k06' size='340' side='right'caption='[[2k06]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2k06]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K06 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K06 FirstGlance]. <br> | <table><tr><td colspan='2'>[[2k06]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K06 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K06 FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k06 OCA], [https://pdbe.org/2k06 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k06 RCSB], [https://www.ebi.ac.uk/pdbsum/2k06 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k06 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k06 OCA], [https://pdbe.org/2k06 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k06 RCSB], [https://www.ebi.ac.uk/pdbsum/2k06 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k06 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/NUSG_ECOLI NUSG_ECOLI] Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNA polymerase (RNAP), probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA and phage lambda N-mediated transcriptional antitermination.<ref>PMID:1532577</ref> <ref>PMID:1547498</ref> <ref>PMID:7505669</ref> <ref>PMID:8422985</ref> <ref>PMID:7868616</ref> <ref>PMID:7761393</ref> <ref>PMID:10383769</ref> <ref>PMID:10820031</ref> <ref>PMID:14973028</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k06 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k06 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | NusG is a conserved regulatory protein that interacts with elongation complexes (ECs) of RNA polymerase, DNA, and RNA to modulate transcription in multiple and sometimes opposite ways. In Escherichia coli, NusG suppresses pausing and increases elongation rate, enhances termination by E. coli rho and phage HK022 Nun protein, and promotes antitermination by lambdaN and in ribosomal RNA operons. We report NMR studies that suggest that E. coli NusG consists of two largely independent N- and C-terminal structural domains, NTD and CTD, respectively. Based on tests of the functions of the NTD and CTD and variants of NusG in vivo and in vitro, we find that NTD alone is sufficient to suppress pausing and enhance transcript elongation in vitro. However, neither domain alone can enhance rho-dependent termination or support antitermination, indicating that interactions of both domains with ECs are required for these processes. We propose that the two domains of NusG mediate distinct interactions with ECs: the NTD interacts with RNA polymerase and the CTD interacts with rho and other regulators, providing NusG with different combinations of interactions to effect different regulatory outcomes. | ||
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| - | Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators.,Mooney RA, Schweimer K, Rosch P, Gottesman M, Landick R J Mol Biol. 2009 Aug 14;391(2):341-58. Epub 2009 Jun 3. PMID:19500594<ref>PMID:19500594</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2k06" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Roesch | + | [[Category: Roesch P]] |
| - | [[Category: Scheckenhofer | + | [[Category: Scheckenhofer U]] |
| - | [[Category: Schweimer | + | [[Category: Schweimer K]] |
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Current revision
Solution structure of the aminoterminal domain of E. coli NusG
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