2kvc
From Proteopedia
(Difference between revisions)
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==Solution structure of the Mycobacterium tuberculosis protein Rv0543c, a member of the DUF3349 superfamily. Seattle Structural Genomics Center for Infectious Disease target MytuD.17112.a== | ==Solution structure of the Mycobacterium tuberculosis protein Rv0543c, a member of the DUF3349 superfamily. Seattle Structural Genomics Center for Infectious Disease target MytuD.17112.a== | ||
| - | <StructureSection load='2kvc' size='340' side='right'caption='[[2kvc | + | <StructureSection load='2kvc' size='340' side='right'caption='[[2kvc]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2kvc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2kvc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Ra Mycobacterium tuberculosis H37Ra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KVC FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kvc OCA], [https://pdbe.org/2kvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kvc RCSB], [https://www.ebi.ac.uk/pdbsum/2kvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kvc ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kvc OCA], [https://pdbe.org/2kvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kvc RCSB], [https://www.ebi.ac.uk/pdbsum/2kvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kvc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A5TZS3_MYCTA A5TZS3_MYCTA] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kvc ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kvc ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The first structure for a member of the DUF3349 (PF11829) family of proteins, Rv0543c from Mycobacterium tuberculosis, has been determined using NMR-based methods and some of its biophysical properties characterized. Rv0543c is a 100 residue, 11.3kDa protein that both size exclusion chromatography and NMR spectroscopy show to be a monomer in solution. The structure of the protein consists of a bundle of five alpha-helices, alpha1 (M1 - Y16), alpha2 (P21 - C33), alpha3 (S37 - G52), alpha4 (G58 - H65) and alpha5 (S72 - G87), held together by a largely conserved group of hydrophobic amino acid side chains. Heteronuclear steady-state {(1)H}-(15)N NOE, T(1), and T(2) values are similar through-out the sequence indicating that the backbones of the five helices are in a single motional regime. The thermal stability of Rv0543c, characterized by circular dichroism spectroscopy, indicates that Rv0543c irreversibly unfolds upon heating with an estimated melting temperature of 62.5 degrees C. While the biological function of Rv0543c is still unknown, the presence of DUF3349 proteins predominately in Mycobacterium and Rhodococcus bacterial species suggests that Rv0543 may have a biological function unique to these bacteria, and consequently, may prove to be an attractive drug target to combat tuberculosis. | ||
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| - | Inaugural structure from the DUF3349 superfamily of proteins, Mycobacterium tuberculosis Rv0543c.,Buchko GW, Phan I, Myler PJ, Terwilliger TC, Kim CY Arch Biochem Biophys. 2011 Feb 15;506(2):150-6. Epub 2010 Dec 6. PMID:21144816<ref>PMID:21144816</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2kvc" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mycobacterium tuberculosis H37Ra]] |
| - | [[Category: Buchko | + | [[Category: Buchko GW]] |
| - | [[Category: Kim | + | [[Category: Kim CY]] |
| - | + | [[Category: Terwilliger TC]] | |
| - | [[Category: Terwilliger | + | |
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Current revision
Solution structure of the Mycobacterium tuberculosis protein Rv0543c, a member of the DUF3349 superfamily. Seattle Structural Genomics Center for Infectious Disease target MytuD.17112.a
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