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2kvk
From Proteopedia
(Difference between revisions)
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==Solution structure of ADF/cofilin (LDCOF) from Leishmania donovani== | ==Solution structure of ADF/cofilin (LDCOF) from Leishmania donovani== | ||
| - | <StructureSection load='2kvk' size='340' side='right'caption='[[2kvk | + | <StructureSection load='2kvk' size='340' side='right'caption='[[2kvk]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2kvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2kvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_donovani Leishmania donovani]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2kd5 2kd5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KVK FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kvk OCA], [https://pdbe.org/2kvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kvk RCSB], [https://www.ebi.ac.uk/pdbsum/2kvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kvk ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kvk OCA], [https://pdbe.org/2kvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kvk RCSB], [https://www.ebi.ac.uk/pdbsum/2kvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kvk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q2QKR1_LEIDO Q2QKR1_LEIDO] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kvk ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kvk ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Leishmania donovani ADF/cofilin (LdCof) is a novel member of ADF/cofilin family. LdCof depolymerizes, but does not co-sediment with, rabbit muscle actin filaments. Its F-actin depolymerizing activity is pH independent. Further, it possesses weak F-actin severing activity. In order to better understand its characteristic properties, we have determined the solution NMR structure of LdCof and have analyzed protein backbone dynamics from (15)N-relaxation measurements. The structure of LdCof possesses a conserved ADF/cofilin fold with a central mixed beta-sheet consisting of six beta-strands which is surrounded by five alpha-helices. LdCof structure has conserved G/F-actin binding site which includes the characteristic long kinked alpha-helix (alpha3). LdCof binds to rabbit muscle ADP-G-actin with 1:1 stoichiometry (K(d) approximately 0.2muM). The F-actin binding site is not well formed and analysis of (15)N-relaxation data shows that residues in the beta4-beta5 loop region and C-terminal are relatively flexible, which seems to be a determinant for the low F-actin severing activity of LdCof. | ||
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| - | Solution structure and dynamics of ADF/cofilin from Leishmania donovani.,Pathak PP, Pulavarti SV, Jain A, Sahasrabuddhe AA, Gupta CM, Arora A J Struct Biol. 2010 Jul 11. PMID:20627129<ref>PMID:20627129</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2kvk" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Leido]] | ||
| - | [[Category: Arora, A]] | ||
| - | [[Category: Gupta, C M]] | ||
| - | [[Category: Jain, A]] | ||
| - | [[Category: Pathak, P P]] | ||
| - | [[Category: Pulavarti, S V]] | ||
| - | [[Category: Sahasrabuddhe, A A]] | ||
| - | [[Category: Adf/cofilin]] | ||
| - | [[Category: Hormone]] | ||
[[Category: Leishmania donovani]] | [[Category: Leishmania donovani]] | ||
| + | [[Category: Arora A]] | ||
| + | [[Category: Gupta CM]] | ||
| + | [[Category: Jain A]] | ||
| + | [[Category: Pathak PP]] | ||
| + | [[Category: Pulavarti SV]] | ||
| + | [[Category: Sahasrabuddhe AA]] | ||
Current revision
Solution structure of ADF/cofilin (LDCOF) from Leishmania donovani
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