2lxq

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Current revision (06:58, 1 May 2024) (edit) (undo)
 
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxq OCA], [https://pdbe.org/2lxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lxq RCSB], [https://www.ebi.ac.uk/pdbsum/2lxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lxq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxq OCA], [https://pdbe.org/2lxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lxq RCSB], [https://www.ebi.ac.uk/pdbsum/2lxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lxq ProSAT]</span></td></tr>
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<div style="background-color:#fffaf0;">
 
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== Publication Abstract from PubMed ==
 
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Neisseria gonorrhoeae is an obligate human pathogen that can escape immune surveillance through antigenic variation of surface structures such as pili. A G-quadruplex-forming (G4) sequence (5'-G(3)TG(3)TTG(3)TG(3)) located upstream of the N. gonorrhoeae pilin expression locus (pilE) is necessary for initiation of pilin antigenic variation, a recombination-based, high-frequency, diversity-generation system. We have determined NMR-based structures of the all parallel-stranded monomeric and 5' end-stacked dimeric pilE G-quadruplexes in monovalent cation-containing solutions. We demonstrate that the three-layered all parallel-stranded monomeric pilE G-quadruplex containing single-residue double-chain reversal loops, which can be modeled without steric clashes into the 3 nt DNA-binding site of RecA, binds and promotes E. coli RecA-mediated strand exchange in vitro. We discuss how interactions between RecA and monomeric pilE G-quadruplex could facilitate the specialized recombination reactions leading to pilin diversification.
 
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RecA-Binding pilE G4 Sequence Essential for Pilin Antigenic Variation Forms Monomeric and 5' End-Stacked Dimeric Parallel G-Quadruplexes.,Kuryavyi V, Cahoon LA, Seifert HS, Patel DJ Structure. 2012 Oct 16. pii: S0969-2126(12)00347-4. doi:, 10.1016/j.str.2012.09.013. PMID:23085077<ref>PMID:23085077</ref>
 
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
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</div>
 
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<div class="pdbe-citations 2lxq" style="background-color:#fffaf0;"></div>
 
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== References ==
 
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<references/>
 
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Current revision

Monomeric PilE G-Quadruplex DNA from Neisseria Gonorrhoeae

PDB ID 2lxq

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