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2mrw

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==Solution Structure of MciZ from Bacillus subtilis==
==Solution Structure of MciZ from Bacillus subtilis==
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<StructureSection load='2mrw' size='340' side='right'caption='[[2mrw]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2mrw' size='340' side='right'caption='[[2mrw]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2mrw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MRW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2mrw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MRW FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mrw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mrw OCA], [https://pdbe.org/2mrw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mrw RCSB], [https://www.ebi.ac.uk/pdbsum/2mrw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mrw ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mrw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mrw OCA], [https://pdbe.org/2mrw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mrw RCSB], [https://www.ebi.ac.uk/pdbsum/2mrw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mrw ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/MCIZ_BACSU MCIZ_BACSU] Blocks Z-ring formation in the mother cell during sporulation by inhibiting the polymerization of FtsZ (PubMed:18284588, PubMed:23237472, PubMed:25848052). Binds to the minus end of FtsZ and functions as a filament-capping protein (PubMed:25848052). At high concentrations, is capable of both capping and sequestration of FtsZ (PubMed:25848052). Decreases the GTPase activity of FtsZ (PubMed:18284588, PubMed:23237472, PubMed:25848052).<ref>PMID:18284588</ref> <ref>PMID:23237472</ref> <ref>PMID:25848052</ref>
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Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division sites, and the constriction of these rings drives cytokinesis. Here we investigate the mechanism by which the Bacillus subtilis cell-division inhibitor, MciZ (mother cell inhibitor of FtsZ), blocks assembly of FtsZ. The X-ray crystal structure reveals that MciZ binds to the C-terminal polymerization interface of FtsZ, the equivalent of the minus end of tubulin. Using in vivo and in vitro assays and microscopy, we show that MciZ, at substoichiometric levels to FtsZ, causes shortening of protofilaments and blocks the assembly of higher-order FtsZ structures. The findings demonstrate an unanticipated capping-based regulatory mechanism for FtsZ.
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FtsZ filament capping by MciZ, a developmental regulator of bacterial division.,Bisson-Filho AW, Discola KF, Castellen P, Blasios V, Martins A, Sforca ML, Garcia W, Zeri AC, Erickson HP, Dessen A, Gueiros-Filho FJ Proc Natl Acad Sci U S A. 2015 Apr 6. pii: 201414242. PMID:25848052<ref>PMID:25848052</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2mrw" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Castellen, P]]
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[[Category: Castellen P]]
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[[Category: Gueiros-Filho, F J]]
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[[Category: Gueiros-Filho FJ]]
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[[Category: Sforca, M L]]
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[[Category: Sforca ML]]
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[[Category: Zeri, A C.M]]
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[[Category: Zeri ACM]]
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[[Category: Cell cycle]]
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[[Category: Ftsz]]
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Current revision

Solution Structure of MciZ from Bacillus subtilis

PDB ID 2mrw

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