4ub9
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ub9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Gulosibacter_molinativorax Gulosibacter molinativorax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UB9 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4ub9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Gulosibacter_molinativorax Gulosibacter molinativorax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UB9 FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ub9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub9 OCA], [https://pdbe.org/4ub9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ub9 RCSB], [https://www.ebi.ac.uk/pdbsum/4ub9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub9 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ub9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ub9 OCA], [https://pdbe.org/4ub9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ub9 RCSB], [https://www.ebi.ac.uk/pdbsum/4ub9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ub9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/G2XLB0_9MICO G2XLB0_9MICO] | [https://www.uniprot.org/uniprot/G2XLB0_9MICO G2XLB0_9MICO] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Molinate is a recalcitrant thiocarbamate used to control grass weeds in rice fields. The recently described molinate hydrolase, from Gulosibacter molinativorax ON4T, plays a key role in the only known molinate degradation pathway ending in the formation of innocuous compounds. Here we report the crystal structure of recombinant molinate hydrolase at 2.27 A. The structure reveals a homotetramer with a single mononuclear metal-dependent active site per monomer. The active site architecture shows similarities with other amidohydrolases and enables us to propose a general acid-base catalysis mechanism for molinate hydrolysis. Molinate hydrolase is unable to degrade bulkier thiocarbamate pesticides such as thiobencarb which is used mostly in rice crops. Using a structural-based approach, we were able to generate a mutant (Arg187Ala) that efficiently degrades thiobencarb. The engineered enzyme is suitable for the development of a broader thiocarbamate bioremediation system. | ||
| - | |||
| - | Structure-guided engineering of molinate hydrolase for the degradation of thiocarbamate pesticides.,Leite JP, Duarte M, Paiva AM, Ferreira-da-Silva F, Matias PM, Nunes OC, Gales L PLoS One. 2015 Apr 23;10(4):e0123430. doi: 10.1371/journal.pone.0123430., eCollection 2015. PMID:25905461<ref>PMID:25905461</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4ub9" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Structural and catalytic characterization of molinate hydrolase
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