|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Physcomitrella patens BBE-like 1 wild-type== | | ==Physcomitrella patens BBE-like 1 wild-type== |
| - | <StructureSection load='6eo4' size='340' side='right' caption='[[6eo4]], [[Resolution|resolution]] 2.90Å' scene=''> | + | <StructureSection load='6eo4' size='340' side='right'caption='[[6eo4]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6eo4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moss Moss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EO4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EO4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6eo4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Physcomitrium_patens Physcomitrium patens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EO4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHYPADRAFT_234241 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3218 Moss])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eo4 OCA], [http://pdbe.org/6eo4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eo4 RCSB], [http://www.ebi.ac.uk/pdbsum/6eo4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eo4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eo4 OCA], [https://pdbe.org/6eo4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eo4 RCSB], [https://www.ebi.ac.uk/pdbsum/6eo4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eo4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A2K1JP57_PHYPA A0A2K1JP57_PHYPA] |
| - | The berberine bridge enzyme from the California poppy Eschscholzia californica (EcBBE) catalyzes the oxidative cyclization of (S)-reticuline to (S)-scoulerine, i.e. the formation of the berberine bridge in the biosynthesis of benzylisoquinoline alkaloids. Interestingly, a large number of BBE-like genes have been identified in plants that lack alkaloid biosynthesis. This finding raised the question of the primordial role of BBE in the plant kingdom, which prompted us to investigate the closest relative of EcBBE in Physcomitrella patens (PpBBE1), the most basal plant harboring a BBE-like gene. Here, we report the biochemical, structural and in vivo characterization of PpBBE1. Our studies revealed that PpBBE1 is structurally and biochemically very similar to EcBBE. In contrast to EcBBE, we found that PpBBE1 catalyzes the oxidation of the disaccharide cellobiose to the corresponding lacton, i.e. PpBBE1 is a cellobiose oxidase. The enzymatic reaction mechanism was characterized by a structure-guided mutagenesis approach that enabled us to assign a catalytic role to amino acid residues in the active site of PpBBE1. In vivo experiments revealed the highest level of PpBBE1 expression in chloronema, the earliest stage of the plant' s life cycle, where carbon metabolism is strongly up-regulated. It was also shown that the enzyme is secreted to the extracellular space, where it may be involved in later steps of cellulose degradation, thereby allowing the moss to make use of cellulose for energy production. Overall, our results suggest that the primordial role of BBE-like enzymes in plants revolved around primary metabolic reactions in carbohydrate utilization. This article is protected by copyright. All rights reserved.
| + | |
| - | | + | |
| - | The single berberine bridge enzyme homolog of Physcomitrella patens is a cellobiose oxidase.,Toplak M, Wiedemann G, Ulicevic J, Daniel B, Hoernstein SNW, Kothe J, Niederhauser J, Reski R, Winkler A, Macheroux P FEBS J. 2018 Apr 6. doi: 10.1111/febs.14458. PMID:29633551<ref>PMID:29633551</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6eo4" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Moss]] | + | [[Category: Large Structures]] |
| - | [[Category: Macheroux, P]] | + | [[Category: Physcomitrium patens]] |
| - | [[Category: Toplak, M]] | + | [[Category: Macheroux P]] |
| - | [[Category: Winkler, A]] | + | [[Category: Toplak M]] |
| - | [[Category: Bbe-like]] | + | [[Category: Winkler A]] |
| - | [[Category: Bi-covalent]]
| + | |
| - | [[Category: Cellobiose]]
| + | |
| - | [[Category: Flavin]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Vao]]
| + | |
