6x46

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Current revision (07:39, 1 May 2024) (edit) (undo)
 
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==NMR solution structure of Asterix/Gtsf1 from mouse (CHHC zinc finger domains)==
==NMR solution structure of Asterix/Gtsf1 from mouse (CHHC zinc finger domains)==
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<StructureSection load='6x46' size='340' side='right'caption='[[6x46]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='6x46' size='340' side='right'caption='[[6x46]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6x46]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X46 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6x46]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X46 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gtsf1, Cue110, Fam112b ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x46 OCA], [https://pdbe.org/6x46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x46 RCSB], [https://www.ebi.ac.uk/pdbsum/6x46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x46 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x46 OCA], [https://pdbe.org/6x46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x46 RCSB], [https://www.ebi.ac.uk/pdbsum/6x46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x46 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/GTSF1_MOUSE GTSF1_MOUSE]] Required for spermatogenesis and is involved in the suppression of retrotransposon transcription in male germ cells.<ref>PMID:19735653</ref>
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[https://www.uniprot.org/uniprot/GTSF1_MOUSE GTSF1_MOUSE] Required for spermatogenesis and is involved in the suppression of retrotransposon transcription in male germ cells.<ref>PMID:19735653</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Piwi-interacting RNA (piRNA) pathway safeguards genomic integrity by silencing transposable elements (transposons) in the germline. While Piwi is the central piRNA factor, others including Asterix/Gtsf1 have also been demonstrated to be critical for effective silencing. Here, using enhanced crosslinking and immunoprecipitation (eCLIP) with a custom informatic pipeline, we show that Asterix/Gtsf1 specifically binds tRNAs in cellular contexts. We determined the structure of mouse Gtsf1 by NMR spectroscopy and identified the RNA-binding interface on the protein's first zinc finger, which was corroborated by biochemical analysis as well as cryo-EM structures of Gtsf1 in complex with co-purifying tRNA. Consistent with the known dependence of long terminal repeat (LTR) retrotransposons on tRNA primers, we demonstrate that LTR retrotransposons are, in fact, preferentially de-repressed in Asterix mutants. Together, these findings link Asterix/Gtsf1, tRNAs, and LTR retrotransposon silencing and suggest that Asterix exploits tRNA dependence to identify transposon transcripts and promote piRNA silencing.
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Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons.,Ipsaro JJ, O'Brien PA, Bhattacharya S, Palmer AG 3rd, Joshua-Tor L Cell Rep. 2021 Mar 30;34(13):108914. doi: 10.1016/j.celrep.2021.108914. PMID:33789107<ref>PMID:33789107</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6x46" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lk3 transgenic mice]]
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[[Category: Mus musculus]]
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[[Category: Bhattacharya, S]]
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[[Category: Bhattacharya S]]
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[[Category: Brien, P A.O]]
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[[Category: Ipsaro JJ]]
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[[Category: III, A G.Palmer]]
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[[Category: Joshua-Tor L]]
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[[Category: Ipsaro, J J]]
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[[Category: O'Brien PA]]
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[[Category: Joshua-Tor, L]]
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[[Category: Palmer III AG]]
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[[Category: Chhc zinc finger]]
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[[Category: Rna binding]]
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[[Category: Rna binding protein]]
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Current revision

NMR solution structure of Asterix/Gtsf1 from mouse (CHHC zinc finger domains)

PDB ID 6x46

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