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| | <StructureSection load='6yhk' size='340' side='right'caption='[[6yhk]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='6yhk' size='340' side='right'caption='[[6yhk]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6yhk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pseudotuberkulosis"_(sic)_pfeiffer_1889 "bacillus pseudotuberkulosis" (sic) pfeiffer 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YHK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yhk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YHK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cnf ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 "Bacillus pseudotuberkulosis" (sic) Pfeiffer 1889])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhk OCA], [http://pdbe.org/6yhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yhk RCSB], [http://www.ebi.ac.uk/pdbsum/6yhk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhk OCA], [https://pdbe.org/6yhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yhk RCSB], [https://www.ebi.ac.uk/pdbsum/6yhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A0N9JNY6_YERPU A0A0N9JNY6_YERPU] |
| - | Cytotoxic necrotizing factors (CNFs) are bacterial single-chain exotoxins that modulate cytokinetic/oncogenic and inflammatory processes through activation of host cell Rho GTPases. To achieve this, they are secreted, bind surface receptors to induce endocytosis and translocate a catalytic unit into the cytosol to intoxicate host cells. A three-dimensional structure that provides insight into the underlying mechanisms is still lacking. Here, we determined the crystal structure of full-length Yersinia pseudotuberculosis CNFY . CNFY consists of five domains (D1-D5), and by integrating structural and functional data, we demonstrate that D1-3 act as export and translocation module for the catalytic unit (D4-5) and for a fused beta-lactamase reporter protein. We further found that D4, which possesses structural similarity to ADP-ribosyl transferases, but had no equivalent catalytic activity, changed its position to interact extensively with D5 in the crystal structure of the free D4-5 fragment. This liberates D5 from a semi-blocked conformation in full-length CNFY , leading to higher deamidation activity. Finally, we identify CNF translocation modules in several uncharacterized fusion proteins, which suggests their usability as a broad-specificity protein delivery tool.
| + | |
| - | | + | |
| - | Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication.,Chaoprasid P, Lukat P, Muhlen S, Heidler T, Gazdag EM, Dong S, Bi W, Ruter C, Kirchenwitz M, Steffen A, Jansch L, Stradal TEB, Dersch P, Blankenfeldt W EMBO J. 2021 Jan 7:e105202. doi: 10.15252/embj.2020105202. PMID:33410511<ref>PMID:33410511</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6yhk" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Blankenfeldt, W]] | + | [[Category: Yersinia pseudotuberculosis]] |
| - | [[Category: Gazdag, E M]] | + | [[Category: Blankenfeldt W]] |
| - | [[Category: Heidler, T V]] | + | [[Category: Gazdag EM]] |
| - | [[Category: Lukat, P]] | + | [[Category: Heidler TV]] |
| - | [[Category: Cnf]] | + | [[Category: Lukat P]] |
| - | [[Category: Cytotoxic necrotizing factor]]
| + | |
| - | [[Category: Deamidase]]
| + | |
| - | [[Category: Putative adp-ribosyltransferase]]
| + | |
| - | [[Category: Rhoa activation]]
| + | |
| - | [[Category: Rhoa modification]]
| + | |
| - | [[Category: Toxin]]
| + | |
