6zh3

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<StructureSection load='6zh3' size='340' side='right'caption='[[6zh3]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='6zh3' size='340' side='right'caption='[[6zh3]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6zh3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZH3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZH3 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6zh3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZH3 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VPS24, DID3, YKL041W, YKL254 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zh3 OCA], [http://pdbe.org/6zh3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zh3 RCSB], [http://www.ebi.ac.uk/pdbsum/6zh3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zh3 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zh3 OCA], [https://pdbe.org/6zh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zh3 RCSB], [https://www.ebi.ac.uk/pdbsum/6zh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zh3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/VPS24_YEAST VPS24_YEAST]] Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment oflate-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. The DID4/VPS2-VPS24 subcomplex is required for the VPS4-dependent dissociation of ESCRT-III.<ref>PMID:11559748</ref> <ref>PMID:12194857</ref> <ref>PMID:9606181</ref>
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[https://www.uniprot.org/uniprot/VPS24_YEAST VPS24_YEAST] Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment oflate-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. The DID4/VPS2-VPS24 subcomplex is required for the VPS4-dependent dissociation of ESCRT-III.<ref>PMID:11559748</ref> <ref>PMID:12194857</ref> <ref>PMID:9606181</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ESCRT-III proteins mediate a range of cellular membrane remodeling activities such as multivesicular body biogenesis, cytokinesis, and viral release. Critical to these processes is the assembly of ESCRT-III subunits into polymeric structures. In this study, we determined the cryo-EM structure of a helical assembly of Saccharomyces cerevisiae Vps24 at 3.2-A resolution and found that Vps24 adopts an elongated open conformation. Vps24 forms a domain-swapped dimer extended into protofilaments that associate into a double-stranded apolar filament. We demonstrate that, upon binding negatively charged lipids, Vps24 homopolymer filaments undergo partial disassembly into shorter filament fragments and oligomers. Upon the addition of Vps24, Vps2, and Snf7, liposomes are deformed into neck and tubular structures by an ESCRT-III heteropolymer coat. The filamentous Vps24 homopolymer assembly structure and interaction studies reveal how Vps24 could introduce unique geometric properties to mixed-type ESCRT-III heteropolymers and contribute to the process of membrane scission events.
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Structure and assembly of ESCRT-III helical Vps24 filaments.,Huber ST, Mostafavi S, Mortensen SA, Sachse C Sci Adv. 2020 Aug 19;6(34):eaba4897. doi: 10.1126/sciadv.aba4897. eCollection, 2020 Aug. PMID:32875105<ref>PMID:32875105</ref>
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==See Also==
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*[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6zh3" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Baker's yeast]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Huber, S T]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Mortensen, S A]]
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[[Category: Huber ST]]
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[[Category: Mostafavi, S]]
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[[Category: Mortensen SA]]
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[[Category: Sachse, C]]
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[[Category: Mostafavi S]]
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[[Category: Filament]]
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[[Category: Sachse C]]
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[[Category: Helical]]
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[[Category: Lipid binding protein]]
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[[Category: Membrane remodeling]]
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Current revision

Cryo-EM structure of ESCRT-III helical Vps24 filaments

PDB ID 6zh3

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