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6zi1

From Proteopedia

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Current revision (07:41, 1 May 2024) (edit) (undo)
 
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<StructureSection load='6zi1' size='340' side='right'caption='[[6zi1]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='6zi1' size='340' side='right'caption='[[6zi1]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6zi1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Haei8 Haei8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZI1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZI1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6zi1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_86-028NP Haemophilus influenzae 86-028NP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZI1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZI1 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">vapD, NTHI0577 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=281310 HAEI8])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zi1 OCA], [http://pdbe.org/6zi1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zi1 RCSB], [http://www.ebi.ac.uk/pdbsum/6zi1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zi1 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zi1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zi1 OCA], [https://pdbe.org/6zi1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zi1 RCSB], [https://www.ebi.ac.uk/pdbsum/6zi1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zi1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/Q4QN95_HAEI8 Q4QN95_HAEI8]] Cleaves ssRNA, mostly between U:A.[PIRNR:PIRNR002882]
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[https://www.uniprot.org/uniprot/Q4QN95_HAEI8 Q4QN95_HAEI8] Cleaves ssRNA, mostly between U:A.[PIRNR:PIRNR002882]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial type II toxin-antitoxin (TA) modules encode a toxic protein that downregulates metabolism and a specific antitoxin that binds and inhibits the toxin during normal growth. In non-typeable Haemophilus influenzae, a common cause of infections in humans, the vapXD locus was found to constitute a functional TA module and contribute to pathogenicity; however, the mode of action of VapD and the mechanism of inhibition by the VapX antitoxin remain unknown. Here, we report the structure of the intact H. influenzae VapXD complex, revealing an unusual 2:1 TA molecular stoichiometry where a Cas2-like homodimer of VapD binds a single VapX antitoxin. VapX consists of an oligonucleotide/oligosaccharide-binding domain that docks into an asymmetrical cavity on the toxin dimer. Structures of isolated VapD further reveal how a symmetrical toxin homodimer adapts to interacting with an asymmetrical antitoxin and suggest how a primordial TA system evolved to become part of CRISPR-Cas immunity systems.
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Structural Basis for Toxin Inhibition in the VapXD Toxin-Antitoxin System.,Bertelsen MB, Senissar M, Nielsen MH, Bisiak F, Cunha MV, Molinaro AL, Daines DA, Brodersen DE Structure. 2020 Oct 16. pii: S0969-2126(20)30373-7. doi:, 10.1016/j.str.2020.10.002. PMID:33096014<ref>PMID:33096014</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6zi1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Haei8]]
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[[Category: Haemophilus influenzae 86-028NP]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Bertelsen, M B]]
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[[Category: Bertelsen MB]]
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[[Category: Bisiak, F]]
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[[Category: Bisiak F]]
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[[Category: Brodersen, D E]]
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[[Category: Brodersen DE]]
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[[Category: Cunha, M V]]
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[[Category: Cunha MV]]
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[[Category: Daines, D A]]
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[[Category: Daines DA]]
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[[Category: Molinaro, A L]]
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[[Category: Molinaro AL]]
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[[Category: Nielsen, M H]]
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[[Category: Nielsen MH]]
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[[Category: Senissar, M]]
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[[Category: Senissar M]]
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[[Category: Hydrolase]]
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[[Category: Nucleic-acid binding protein]]
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[[Category: Rnase]]
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[[Category: Toxin]]
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[[Category: Toxin-antitoxin]]
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[[Category: Vapxd]]
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Current revision

Crystal structure of the isolated H. influenzae VapD toxin (D7N mutant)

PDB ID 6zi1

Drag the structure with the mouse to rotate

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