1sg5
From Proteopedia
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==Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination== | ==Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination== | ||
| - | <StructureSection load='1sg5' size='340' side='right'caption='[[1sg5 | + | <StructureSection load='1sg5' size='340' side='right'caption='[[1sg5]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1sg5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1sg5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SG5 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sg5 OCA], [https://pdbe.org/1sg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sg5 RCSB], [https://www.ebi.ac.uk/pdbsum/1sg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sg5 ProSAT], [https://www.topsan.org/Proteins/BSGI/1sg5 TOPSAN]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sg5 OCA], [https://pdbe.org/1sg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sg5 RCSB], [https://www.ebi.ac.uk/pdbsum/1sg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sg5 ProSAT], [https://www.topsan.org/Proteins/BSGI/1sg5 TOPSAN]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/ROF_ECOLI ROF_ECOLI] Suppresses temperature-sensitive mutations in essential genes by modulating rho-dependent transcription termination. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sg5 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sg5 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Rho-dependent transcription termination is an essential process for the regulation of bacterial gene expression. Thus far, only two Rho-specific inhibitors of bacterial transcription termination have been described, the psu protein from the satellite bacteriophage P4 and YaeO from Escherichia coli. Here, we report the solution structure of YaeO, the first of a Rho-specific inhibitor of transcription termination. YaeO is an acidic protein composed of an N-terminal helix and a seven-stranded beta sandwich. NMR chemical shift perturbation experiments revealed that YaeO binds proximal to the primary nucleic acid binding site of Rho. Based on the NMR titrations, a docked model of the YaeO-Rho complex was calculated. These results suggest that YaeO binds outside the Rho hexamer, acting as a competitive inhibitor of RNA binding. In vitro gel shift assays confirmed the inhibition of nucleic acid binding to Rho. Site-directed mutagenesis showed that the negative character of YaeO is essential for its function in vivo. | ||
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| - | Solution structure of YaeO, a Rho-specific inhibitor of transcription termination.,Gutierrez P, Kozlov G, Gabrielli L, Elias D, Osborne MJ, Gallouzi IE, Gehring K J Biol Chem. 2007 Aug 10;282(32):23348-53. Epub 2007 Jun 11. PMID:17565995<ref>PMID:17565995</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1sg5" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Escherichia coli]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | + | [[Category: Gehring K]] | |
| - | [[Category: Gehring | + | [[Category: Gutierrez P]] |
| - | [[Category: Gutierrez | + | |
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Revision as of 08:31, 1 May 2024
Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination
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