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1t2s
From Proteopedia
(Difference between revisions)
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==Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain== | ==Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain== | ||
| - | <StructureSection load='1t2s' size='340' side='right'caption='[[1t2s | + | <StructureSection load='1t2s' size='340' side='right'caption='[[1t2s]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1t2s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1t2s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2S FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2s OCA], [https://pdbe.org/1t2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2s RCSB], [https://www.ebi.ac.uk/pdbsum/1t2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2s ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2s OCA], [https://pdbe.org/1t2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2s RCSB], [https://www.ebi.ac.uk/pdbsum/1t2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/AGO2_DROME AGO2_DROME] Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger.<ref>PMID:11498593</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2s ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2s ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs. | ||
| - | |||
| - | Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.,Lingel A, Simon B, Izaurralde E, Sattler M Nat Struct Mol Biol. 2004 Jun;11(6):576-7. Epub 2004 May 23. PMID:15156196<ref>PMID:15156196</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1t2s" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Drosophila melanogaster]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Izaurralde | + | [[Category: Izaurralde E]] |
| - | [[Category: Lingel | + | [[Category: Lingel A]] |
| - | [[Category: Sattler | + | [[Category: Sattler M]] |
| - | [[Category: Simon | + | [[Category: Simon B]] |
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Revision as of 08:37, 1 May 2024
Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain
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