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1rxw
From Proteopedia
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[[Image:1rxw.gif|left|200px]] | [[Image:1rxw.gif|left|200px]] | ||
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'''Crystal structure of A. fulgidus FEN-1 bound to DNA''' | '''Crystal structure of A. fulgidus FEN-1 bound to DNA''' | ||
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[[Category: Yelent, B.]] | [[Category: Yelent, B.]] | ||
[[Category: 3' flap binding site]] | [[Category: 3' flap binding site]] | ||
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| - | [[Category: | + | [[Category: Helix-3 turn-helix]] |
| - | [[Category: | + | [[Category: Hydrophobic wedge]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:02:24 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:02, 3 May 2008
Crystal structure of A. fulgidus FEN-1 bound to DNA
Overview
Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
About this Structure
1RXW is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:14718165 Page seeded by OCA on Sat May 3 08:02:24 2008
