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Publication Abstract from PubMed

Guanylate cyclase activating protein-5 (GCAP5) in zebrafish photoreceptors promotes the activation of membrane receptor retinal guanylate cyclase (GC-E). Previously, we showed the R22A mutation in GCAP5 (GCAP5(R22A)) abolishes dimerization of GCAP5 and activates GC-E by more than 3-fold compared to that of wild-type GCAP5 (GCAP5(WT)). Here, we present ITC, NMR, and functional analysis of GCAP5(R22A) to understand how R22A causes a decreased dimerization affinity and increased cyclase activation. ITC experiments reveal GCAP5(R22A) binds a total of 3 Ca(2+), including two sites in the nanomolar range followed by a single micromolar site. The two nanomolar sites in GCAP5(WT) were not detected by ITC, suggesting that R22A may affect the binding of Ca(2+) to these sites. The NMR-derived structure of GCAP5(R22A) is overall similar to that of GCAP5(WT) (RMSD = 2.3 A), except for local differences near R22A (Q19, W20, Y21, and K23) and an altered orientation of the C-terminal helix near the N-terminal myristate. GCAP5(R22A) lacks an intermolecular salt bridge between R22 and D71 that may explain the weakened dimerization. We present a structural model of GCAP5 bound to GC-E in which the R22 side-chain contacts exposed hydrophobic residues in GC-E. Cyclase assays suggest that GC-E binds to GCAP5(R22A) with approximately 25% higher affinity compared to GCAP5(WT), consistent with more favorable hydrophobic contact by R22A that may help explain the increased cyclase activation.

NMR Structure of Retinal Guanylate Cyclase Activating Protein 5 (GCAP5) with R22A Mutation That Abolishes Dimerization and Enhances Cyclase Activation.,Cudia DL, Ahoulou EO, Bej A, Janssen AN, Scholten A, Koch KW, Ames JB Biochemistry. 2024 Apr 25. doi: 10.1021/acs.biochem.4c00046. PMID:38662574^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.