This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2jdg

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Affilin based on HUMAN GAMMA-B CRYSTALLIN

Structural highlights

2jdg is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

CRGB_HUMAN Zonular cataract;Anterior polar cataract;Total congenital cataract. The disease is caused by mutations affecting the gene represented in this entry.^[1]

Function

CRGB_HUMAN Crystallins are the dominant structural components of the vertebrate eye lens.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The concept of novel binding proteins as an alternative to antibodies has undergone rapid development and is now ready for practical use in a wide range of applications. Alternative binding proteins, based on suitable scaffolds with desirable properties, are selected from combinatorial libraries in vitro. Here, we describe an approach using a beta-sheet of human gamma-B-crystallin to generate a universal binding site through randomization of eight solvent-exposed amino acid residues selected according to structural and sequence analyses. Specific variants, so-called Affilin, have been isolated from a phage display library against a variety of targets that differ considerably in size and structure. The isolated Affilin variants can be produced in Escherichia coli as soluble proteins and have a high level of thermodynamic stability. The crystal structures of the human wild-type gamma-B-crystallin and a selected Affilin variant have been determined to 1.7 A and 2.0 A resolution, respectively. Comparison of the two molecules indicates that the human gamma-B-crystallin tolerates amino acid exchanges with no major structural change. We conclude that the intrinsically stable and easily expressed gamma-B-crystallin provides a suitable framework for the generation of novel binding molecules.

Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein.,Ebersbach H, Fiedler E, Scheuermann T, Fiedler M, Stubbs MT, Reimann C, Proetzel G, Rudolph R, Fiedler U J Mol Biol. 2007 Sep 7;372(1):172-85. Epub 2007 Jun 22. PMID:17628592^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ AlFadhli S, Abdelmoaty S, Al-Hajeri A, Behbehani A, Alkuraya F. Novel crystallin gamma B mutations in a Kuwaiti family with autosomal dominant congenital cataracts reveal genetic and clinical heterogeneity. Mol Vis. 2012;18:2931-6. Epub 2012 Dec 9. PMID:23288985
↑ Ebersbach H, Fiedler E, Scheuermann T, Fiedler M, Stubbs MT, Reimann C, Proetzel G, Rudolph R, Fiedler U. Affilin-novel binding molecules based on human gamma-B-crystallin, an all beta-sheet protein. J Mol Biol. 2007 Sep 7;372(1):172-85. Epub 2007 Jun 22. PMID:17628592 doi:10.1016/j.jmb.2007.06.045

1 Structural highlights
2 Disease
3 Function
4 Evolutionary Conservation
5 Publication Abstract from PubMed
6 See Also
7 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jdg"

@@ Line 3: / Line 3: @@
 <StructureSection load='2jdg' size='340' side='right'caption='[[2jdg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jdg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JDG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jdg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JDG FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1leu|1leu]], [[1myv|1myv]], [[1myx|1myx]], [[1myy|1myy]], [[1mz1|1mz1]], [[1mz2|1mz2]], [[1mz3|1mz3]], [[2jdf|2jdf]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jdg OCA], [https://pdbe.org/2jdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jdg RCSB], [https://www.ebi.ac.uk/pdbsum/2jdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jdg ProSAT]</span></td></tr>
 </table>
+== Disease ==
+[https://www.uniprot.org/uniprot/CRGB_HUMAN CRGB_HUMAN] Zonular cataract;Anterior polar cataract;Total congenital cataract. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:23288985</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/CRGB_HUMAN CRGB_HUMAN] Crystallins are the dominant structural components of the vertebrate eye lens.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 37: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Ebersbach, H]]
+[[Category: Ebersbach H]]
-[[Category: Fiedler, E]]
+[[Category: Fiedler E]]
-[[Category: Fiedler, M]]
+[[Category: Fiedler M]]
-[[Category: Fiedler, U]]
+[[Category: Fiedler U]]
-[[Category: Proetzel, G]]
+[[Category: Proetzel G]]
-[[Category: Reimann, C]]
+[[Category: Reimann C]]
-[[Category: Rudolph, R]]
+[[Category: Rudolph R]]
-[[Category: Scheuermann, T]]
+[[Category: Scheuermann T]]
-[[Category: Stubbs, M T]]
+[[Category: Stubbs MT]]
-[[Category: Affilin]]
-[[Category: Artificial binding protein]]
-[[Category: Eye lens protein]]
-[[Category: Gamma crystallin]]
-[[Category: Oxidation]]
-[[Category: Phosphorylation]]
-[[Category: Polymorphism]]
-[[Category: Structural protein]]

2jdg

From Proteopedia

Current revision

Affilin based on HUMAN GAMMA-B CRYSTALLIN

Structural highlights

Disease

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox