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2k77

From Proteopedia

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NMR solution structure of the Bacillus subtilis ClpC N-domain

Structural highlights

2k77 is a 1 chain structure with sequence from Bacillus subtilis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CLPC_BACSU Competence gene repressor; required for cell growth at high temperature. Negative regulator of comK expression. May interact with MecA to negatively regulate comK.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The AAA(+) (ATPases associated with a variety of cellular activities) superfamily protein ClpC is a key regulator of cell development in Bacillus subtilis. As part of a large oligomeric complex, ClpC controls an array of cellular processes by recognizing, unfolding, and providing misfolded and aggregated proteins as substrates for the ClpP peptidase. ClpC is unique compared to other HSP100/Clp proteins, as it requires an adaptor protein for all fundamental activities. The NMR solution structure of the N-terminal repeat domain of ClpC (N-ClpCR) comprises two structural repeats of a four-helix motif. NMR experiments used to map the MecA adaptor protein interaction surface of N-ClpCR reveal that regions involved in the interaction possess conformational flexibility and conformational exchange on the microsecond-to-millisecond timescale. The electrostatic surface of N-ClpCR differs substantially from the N-domain of Escherichia coli ClpA and ClpB, suggesting that the electrostatic surface characteristics of HSP100/Clp N-domains may play a role in adaptor protein and substrate interaction specificity, and perhaps contribute to the unique adaptor protein requirement of ClpC.

Structural and motional contributions of the Bacillus subtilis ClpC N-domain to adaptor protein interactions.,Kojetin DJ, McLaughlin PD, Thompson RJ, Dubnau D, Prepiak P, Rance M, Cavanagh J J Mol Biol. 2009 Apr 3;387(3):639-52. Epub 2009 Jan 30. PMID:19361434^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kojetin DJ, McLaughlin PD, Thompson RJ, Dubnau D, Prepiak P, Rance M, Cavanagh J. Structural and motional contributions of the Bacillus subtilis ClpC N-domain to adaptor protein interactions. J Mol Biol. 2009 Apr 3;387(3):639-52. Epub 2009 Jan 30. PMID:19361434 doi:10.1016/j.jmb.2009.01.046

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2k77"

@@ Line 1: / Line 1: @@
 ==NMR solution structure of the Bacillus subtilis ClpC N-domain==
-<StructureSection load='2k77' size='340' side='right'caption='[[2k77]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
+<StructureSection load='2k77' size='340' side='right'caption='[[2k77]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2k77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K77 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2k77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K77 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">clpC, mecB, BSU00860 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k77 OCA], [https://pdbe.org/2k77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k77 RCSB], [https://www.ebi.ac.uk/pdbsum/2k77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k77 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/CLPC_BACSU CLPC_BACSU]] Competence gene repressor; required for cell growth at high temperature. Negative regulator of comK expression. May interact with MecA to negatively regulate comK.
+[https://www.uniprot.org/uniprot/CLPC_BACSU CLPC_BACSU] Competence gene repressor; required for cell growth at high temperature. Negative regulator of comK expression. May interact with MecA to negatively regulate comK.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Vibrio subtilis ehrenberg 1835]]
+[[Category: Bacillus subtilis]]
 [[Category: Large Structures]]
-[[Category: Cavanagh, J]]
+[[Category: Cavanagh J]]
-[[Category: Kojetin, D J]]
+[[Category: Kojetin DJ]]
-[[Category: McLaughlin, P D]]
+[[Category: McLaughlin PD]]
-[[Category: Rance, M]]
+[[Category: Rance M]]
-[[Category: Thompson, R J]]
+[[Category: Thompson RJ]]
-[[Category: Chaperone]]
-[[Category: Chaperone/protease]]
-[[Category: Competence]]
-[[Category: Hsp100/clp/aaa+]]
-[[Category: N-clpcr]]
-[[Category: N-domain]]
-[[Category: Protein binding]]

2k77

From Proteopedia

Current revision

NMR solution structure of the Bacillus subtilis ClpC N-domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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