1uoh
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(New page: 200px<br /> <applet load="1uoh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uoh, resolution 2.00Å" /> '''HUMAN GANKYRIN'''<b...)
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Revision as of 17:30, 12 November 2007
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HUMAN GANKYRIN
Overview
Gankyrin is a 25-kDa hepatocellular carcinoma-associated protein that, mediates protein-protein interactions in cell cycle control and protein, degradation. It has been reported to form complexes with cyclin-dependent, kinase 4, retinoblastoma protein, the S6b ATPase subunit of the 19 S, regulator of the 26 S proteasome, and Mdm2, an E3 ubiquitin ligase, involved in p53 degradation. It is the first protein described to bind, both to the 26 S proteasome and to proteins in other complexes containing, cyclin-dependent kinase(s) and p53 ubiquitylating activities, thus, providing a mechanism for delivering cell cycle regulating machinery and, ubiquitylated substrates to the proteasome for degradation. Gankyrin, contains a 33-residue motif known as the ankyrin repeat that occurs five, and a half to six times in the sequence. As a step toward understanding, gankyrin interactions with its protein partners we have determined its, three-dimensional crystal structure to 2.0-A resolution. It reveals that, the entire 226-residue gankyrin polypeptide folds into seven ankyrin, repeat elements. The ankyrin repeats, consisting of an antiparallel, beta-hairpin followed by a perpendicularly oriented helix-loop-helix, pack, side-by-side, creating an extended curved structure with a groove running, across the long concave surface. Comparison with the structures of other, ankyrin repeat proteins suggests that interactions with partner proteins, are mediated by residues situated on this concave surface.
About this Structure
1UOH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53., Krzywda S, Brzozowski AM, Higashitsuji H, Fujita J, Welchman R, Dawson S, Mayer RJ, Wilkinson AJ, J Biol Chem. 2004 Jan 9;279(2):1541-5. Epub 2003 Oct 22. PMID:14573599
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