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| | <StructureSection load='2uuz' size='340' side='right'caption='[[2uuz]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='2uuz' size='340' side='right'caption='[[2uuz]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2uuz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2uuz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2uv1|2uv1]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uuz OCA], [https://pdbe.org/2uuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uuz RCSB], [https://www.ebi.ac.uk/pdbsum/2uuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uuz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uuz OCA], [https://pdbe.org/2uuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uuz RCSB], [https://www.ebi.ac.uk/pdbsum/2uuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uuz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/VGAM_LAMBD VGAM_LAMBD]] Gene GAM protein inhibits the host recBC-coded nuclease and thus promotes the late, rolling-circle mode of lambda DNA replication.
| + | [https://www.uniprot.org/uniprot/GAM_LAMBD GAM_LAMBD] Binds to host RecBCD nuclease and inhibits it thereby protecting the viral DNA against recBCD mediated degradation.<ref>PMID:17544443</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacteriophage lambda]] | + | [[Category: Escherichia virus Lambda]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cook, N]] | + | [[Category: Cook N]] |
| - | [[Category: Court, R I]] | + | [[Category: Court RI]] |
| - | [[Category: Saikrishnan, K]] | + | [[Category: Saikrishnan K]] |
| - | [[Category: Wigley, D B]] | + | [[Category: Wigley DB]] |
| - | [[Category: Inhibitor]]
| + | |
| - | [[Category: Nuclease inhibitor]]
| + | |
| - | [[Category: Putative dna mimic]]
| + | |
| - | [[Category: Recbcd inhibitor]]
| + | |
| Structural highlights
Function
GAM_LAMBD Binds to host RecBCD nuclease and inhibits it thereby protecting the viral DNA against recBCD mediated degradation.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In Escherichia coli, RecBCD processes double-stranded DNA breaks during the initial stages of homologous recombination. RecBCD contains helicase and nuclease activities, and unwinds and digests the blunt-ended DNA until a specific eight-nucleotide sequence, Chi, is encountered. Chi modulates the nuclease activity of RecBCD and results in a resected DNA end, which is a substrate for RecA during subsequent steps in recombination. RecBCD also acts as a defence mechanism against bacteriophage infection by digesting linear viral DNA present during virus replication or resulting from the action of restriction endonucleases. To avoid this fate, bacteriophage lambda encodes the gene Gam whose product is an inhibitor of RecBCD. Gam has been shown to bind to RecBCD and inhibit its helicase and nuclease activities. We show that Gam inhibits RecBCD by preventing it from binding DNA. We have solved the crystal structure of Gam from two different crystal forms. Using the published crystal structure of RecBCD in complex with DNA we suggest models for the molecular mechanism of Gam-mediated inhibition of RecBCD. We also propose that Gam could be a mimetic of single-stranded, and perhaps also double-stranded, DNA.
The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition.,Court R, Cook N, Saikrishnan K, Wigley D J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:17544443[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Court R, Cook N, Saikrishnan K, Wigley D. The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition. J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:17544443 doi:10.1016/j.jmb.2007.05.037
- ↑ Court R, Cook N, Saikrishnan K, Wigley D. The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition. J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:17544443 doi:10.1016/j.jmb.2007.05.037
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