2wzm

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of a mycobacterium aldo-keto reductase in its apo and liganded form

Structural highlights

2wzm is a 2 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.64Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Y2407_MYCS2

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Aldo-keto reductases (AKRs) are a large superfamily of NADPH-dependent enzymes that catalyze the reduction of aldehydes, aldoses, dicarbonyls, steroids, and monosaccharides. While their precise physiological role is generally unknown, AKRs are nevertheless involved in the detoxification of a broad range of toxic metabolites. Mycobacteria contain a number of AKRs, the majority of which are uncharacterised. Here, we report the 1.9 and 1.6 A resolution structures of the apoenzyme and NADPH-bound forms, respectively, of an AKR (MSMEG_2407) from Mycobacterium smegmatis, a close homologue of the M. tuberculosis enzyme Rv2971, whose function is essential to this bacterium. MSMEG_2407 adopted the triosephosphate isomerase (alpha/beta)(8)-barrel fold exhibited by other AKRs. MSMEG_2407 (AKR5H1) bound NADPH via an induced-fit mechanism, in which the NADPH was ligated in an extended fashion. Polar-mediated interactions dominated the interactions with the cofactor, which is atypical of the mode of NADPH binding within the AKR family. Moreover, the nicotinamide ring of NADPH was disordered, and this was attributed to the lack of an "AKR-conserved" bulky residue within the nicotinamide-binding cavity of MSMEG_2407. Enzymatic characterisation of MSMEG_2407 and Rv2971 identified dicarbonyls as a preferred substrate family for hydrolysis, and the frontline antituberculosis drug isoniazid (INH) was shown to inhibit the enzyme activity of both recombinant MSMEG_2407 and Rv2971. However, differences between the affinities of MSMEG_2407 and Rv2971 for dicarbonyls and INH were observed, and this was attributable to amino acid substitutions within the cofactor- and substrate-binding sites. The structures of MSMEG_2407 and the accompanying biochemical characterisation of MSMEG_2407 and Rv2971 provide insight into the structure and function of AKRs from mycobacteria.

Crystal structure and comparative functional analyses of a Mycobacterium aldo-keto reductase.,Scoble J, McAlister AD, Fulton Z, Troy S, Byres E, Vivian JP, Brammananth R, Wilce MC, Le Nours J, Zaker-Tabrizi L, Coppel RL, Crellin PK, Rossjohn J, Beddoe T J Mol Biol. 2010 Apr 23;398(1):26-39. Epub 2010 Feb 25. PMID:20188740^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Scoble J, McAlister AD, Fulton Z, Troy S, Byres E, Vivian JP, Brammananth R, Wilce MC, Le Nours J, Zaker-Tabrizi L, Coppel RL, Crellin PK, Rossjohn J, Beddoe T. Crystal structure and comparative functional analyses of a Mycobacterium aldo-keto reductase. J Mol Biol. 2010 Apr 23;398(1):26-39. Epub 2010 Feb 25. PMID:20188740 doi:10.1016/j.jmb.2010.02.021

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wzm"

@@ Line 3: / Line 3: @@
 <StructureSection load='2wzm' size='340' side='right'caption='[[2wzm]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wzm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WZM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wzm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WZM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA7:[(2R,3R,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3-HYDROXY-4-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+[(2R,3S,4S)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NA7</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wzt|2wzt]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA7:[(2R,3R,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3-HYDROXY-4-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+[(2R,3S,4S)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NA7</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Morphine_6-dehydrogenase Morphine 6-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.218 1.1.1.218] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wzm OCA], [https://pdbe.org/2wzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wzm RCSB], [https://www.ebi.ac.uk/pdbsum/2wzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wzm ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Y2407_MYCS2 Y2407_MYCS2]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 37: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Morphine 6-dehydrogenase]]
+[[Category: Mycolicibacterium smegmatis MC2 155]]
-[[Category: Mycs2]]
+[[Category: Beddoe T]]
-[[Category: Beddoe, T]]
+[[Category: Brammananth R]]
-[[Category: Brammananth, R]]
+[[Category: Byres E]]
-[[Category: Byres, E]]
+[[Category: Coppel RL]]
-[[Category: Coppel, R L]]
+[[Category: Crellin PK]]
-[[Category: Crellin, P K]]
+[[Category: Fulton Z]]
-[[Category: Fulton, Z]]
+[[Category: Le Nours J]]
-[[Category: McAlister, A D]]
+[[Category: McAlister AD]]
-[[Category: Nours, J Le]]
+[[Category: Rossjohn J]]
-[[Category: Rossjohn, J]]
+[[Category: Scoble J]]
-[[Category: Scoble, J]]
+[[Category: Troy S]]
-[[Category: Troy, S]]
+[[Category: Vivian JP]]
-[[Category: Vivian, J P]]
+[[Category: Wilce MCJ]]
-[[Category: Wilce, M C.J]]
+[[Category: Zaker-Tabrizi L]]
-[[Category: Zaker-Tabrizi, L]]
-[[Category: Oxidoreductase]]

2wzm

From Proteopedia

Current revision

Crystal structure of a mycobacterium aldo-keto reductase in its apo and liganded form

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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