1s0v

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[[Image:1s0v.gif|left|200px]]
[[Image:1s0v.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1s0v |SIZE=350|CAPTION= <scene name='initialview01'>1s0v</scene>, resolution 3.2&Aring;
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The line below this paragraph, containing "STRUCTURE_1s0v", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= 1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])
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-->
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|DOMAIN=
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{{STRUCTURE_1s0v| PDB=1s0v | SCENE= }}
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|RELATEDENTRY=[[1h38|1H38]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s0v OCA], [http://www.ebi.ac.uk/pdbsum/1s0v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s0v RCSB]</span>
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}}
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'''Structural basis for substrate selection by T7 RNA polymerase'''
'''Structural basis for substrate selection by T7 RNA polymerase'''
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[[Category: Vassylyev, D G.]]
[[Category: Vassylyev, D G.]]
[[Category: Yokoyama, S.]]
[[Category: Yokoyama, S.]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rna]]
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[[Category: Rna]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: t7 rna polymerase]]
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[[Category: T7 rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:09:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:35:45 2008''
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Revision as of 05:09, 3 May 2008

Image:1s0v.gif

Template:STRUCTURE 1s0v

Structural basis for substrate selection by T7 RNA polymerase


Overview

The mechanism by which nucleotide polymerases select the correct substrate is of fundamental importance to the fidelity of DNA replication and transcription. During the nucleotide addition cycle, pol I DNA polymerases undergo the transition from a catalytically inactive "open" to an active "closed" conformation. All known determinants of substrate selection are associated with the "closed" state. To elucidate if this mechanism is conserved in homologous single subunit RNA polymerases (RNAPs), we have determined the structure of T7 RNAP elongation complex with the incoming substrate analog. Surprisingly, the substrate specifically binds to RNAP in the "open" conformation, where it is base paired with the acceptor template base, while Tyr639 provides discrimination of ribose versus deoxyribose substrates. The structure therefore suggests a novel mechanism, in which the substrate selection occurs prior to the isomerization to the catalytically active conformation. Modeling of multisubunit RNAPs suggests that this mechanism might be universal for all RNAPs.

About this Structure

1S0V is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.

Reference

Structural basis for substrate selection by t7 RNA polymerase., Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG, Cell. 2004 Feb 6;116(3):381-91. PMID:15016373 Page seeded by OCA on Sat May 3 08:09:16 2008

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