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4ynh

From Proteopedia

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Current revision

Structure of the C. elegans SAS-5 Implico dimerization domain

Structural highlights

4ynh is a 2 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAS5_CAEEL Required for centrosome duplication. Essential for daughter-centriole formation. Requires both maternal and partenal expression, suggesting that it regulates centriole duplication during both spermatogenesis and early embryogenesis.^[1] ^[2]

Publication Abstract from PubMed

Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In C. elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional homologs across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear. Here, we combine biophysical and structural analysis to uncover the architecture of SAS-5 and examine its functional implications in vivo. Our work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo.

The protein SAS-5 forms large oligomeric assemblies critical for centriole formation.,Rogala KB, Dynes NJ, Hatzopoulos GN, Yan J, Pong SK, Robinson CV, Deane CM, Gonczy P, Vakonakis I Elife. 2015 May 29;4. doi: 10.7554/eLife.07410. PMID:26023830^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Delattre M, Leidel S, Wani K, Baumer K, Bamat J, Schnabel H, Feichtinger R, Schnabel R, Gonczy P. Centriolar SAS-5 is required for centrosome duplication in C. elegans. Nat Cell Biol. 2004 Jul;6(7):656-64. PMID:15232593 doi:http://dx.doi.org/10.1038/ncb1146
↑ Dammermann A, Muller-Reichert T, Pelletier L, Habermann B, Desai A, Oegema K. Centriole assembly requires both centriolar and pericentriolar material proteins. Dev Cell. 2004 Dec;7(6):815-29. PMID:15572125 doi:S1534580704003806
↑ Rogala KB, Dynes NJ, Hatzopoulos GN, Yan J, Pong SK, Robinson CV, Deane CM, Gonczy P, Vakonakis I. The protein SAS-5 forms large oligomeric assemblies critical for centriole formation. Elife. 2015 May 29;4. doi: 10.7554/eLife.07410. PMID:26023830 doi:http://dx.doi.org/10.7554/eLife.07410

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ynh"

Categories: Caenorhabditis elegans | Large Structures | Hatzopoulos GN | Rogala KB | Vakonakis I

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4ynh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YNH FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ynh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynh OCA], [https://pdbe.org/4ynh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ynh RCSB], [https://www.ebi.ac.uk/pdbsum/4ynh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynh ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ynh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynh OCA], [https://pdbe.org/4ynh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ynh RCSB], [https://www.ebi.ac.uk/pdbsum/4ynh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynh ProSAT]</span></td></tr>
 </table>
 == Function ==

4ynh

From Proteopedia

Current revision

Structure of the C. elegans SAS-5 Implico dimerization domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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