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5abb

From Proteopedia

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Visualization of a polytopic membrane protein during SecY-mediated membrane insertion

5abb, resolution 8.00Å

Structural highlights

5abb is a 3 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 8Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SECY_ECOLI The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. SecY is required to insert newly synthesized SecY into the inner membrane. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true, overexpression also results in FtsH-mediated degradation of SecY.[HAMAP-Rule:MF_01465]

Publication Abstract from PubMed

The biogenesis of polytopic membrane proteins occurs co-translationally on ribosomes that are tightly bound to a membrane-embedded protein-conducting channel: the Sec-complex. The path that is followed by nascent proteins inside the ribosome and the Sec-complex is relatively well established; however, it is not clear what the fate of the N-terminal transmembrane domains (TMDs) of polytopic membrane proteins is when the C-terminal TMDs domains are not yet synthesized. Here, we present the sub-nanometer cryo-electron microscopy structure of an in vivo generated ribosome-SecY complex that carries a membrane insertion intermediate of proteorhodopsin (PR). The structure reveals a pre-opened Sec-complex and the first two TMDs of PR already outside the SecY complex directly in front of its proposed lateral gate. Thus, our structure is in agreement with positioning of N-terminal TMDs at the periphery of SecY, and in addition, it provides clues for the molecular mechanism underlying membrane protein topogenesis.

Visualization of a polytopic membrane protein during SecY-mediated membrane insertion.,Bischoff L, Wickles S, Berninghausen O, van der Sluis EO, Beckmann R Nat Commun. 2014 Jun 10;5:4103. doi: 10.1038/ncomms5103. PMID:24912953^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bischoff L, Wickles S, Berninghausen O, van der Sluis EO, Beckmann R. Visualization of a polytopic membrane protein during SecY-mediated membrane insertion. Nat Commun. 2014 Jun 10;5:4103. doi: 10.1038/ncomms5103. PMID:24912953 doi:http://dx.doi.org/10.1038/ncomms5103

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5abb"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5abb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ABB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ABB FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5abb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5abb OCA], [https://pdbe.org/5abb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5abb RCSB], [https://www.ebi.ac.uk/pdbsum/5abb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5abb ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5abb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5abb OCA], [https://pdbe.org/5abb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5abb RCSB], [https://www.ebi.ac.uk/pdbsum/5abb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5abb ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 20: / Line 21: @@
 ==See Also==
 *[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
-*[[Preprotein translocase|Preprotein translocase]]
+*[[Preprotein translocase 3D structures|Preprotein translocase 3D structures]]
 == References ==
 <references/>

5abb

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Current revision

Visualization of a polytopic membrane protein during SecY-mediated membrane insertion

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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