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| | <StructureSection load='6eyy' size='340' side='right'caption='[[6eyy]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='6eyy' size='340' side='right'caption='[[6eyy]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6eyy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_phage_73 Streptococcus phage 73]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EYY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6eyy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_73 Streptococcus phage 73]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EYY FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6eyx|6eyx]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eyy OCA], [http://pdbe.org/6eyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eyy RCSB], [http://www.ebi.ac.uk/pdbsum/6eyy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eyy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eyy OCA], [https://pdbe.org/6eyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eyy RCSB], [https://www.ebi.ac.uk/pdbsum/6eyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eyy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A1S5PRR0_9CAUD A0A1S5PRR0_9CAUD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Streptococcus phage 73]] | | [[Category: Streptococcus phage 73]] |
| - | [[Category: Amigues, B]] | + | [[Category: Amigues B]] |
| - | [[Category: Cambillau, C]] | + | [[Category: Cambillau C]] |
| - | [[Category: Moineau, S]] | + | [[Category: Moineau S]] |
| - | [[Category: Anti-crispr]]
| + | |
| - | [[Category: Dna binding]]
| + | |
| - | [[Category: Hth fold]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
A0A1S5PRR0_9CAUD
Publication Abstract from PubMed
CRISPR-Cas systems are bacterial anti-viral systems, and bacterial viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs can also fine-tune the activity of CRISPR-based genome-editing tools. While Acrs are prevalent in phages capable of lying dormant in a CRISPR-carrying host, their orthologs have been observed only infrequently in virulent phages. Here we identify AcrIIA6, an Acr encoded in 33% of virulent Streptococcus thermophilus phage genomes. The X-ray structure of AcrIIA6 displays some features unique to this Acr family. We compare the activity of AcrIIA6 to those of other Acrs, including AcrIIA5 (also from S. thermophilus phages), and characterize their effectiveness against a range of CRISPR-Cas systems. Finally, we demonstrate that both Acr families from S. thermophilus phages inhibit Cas9-mediated genome editing of human cells.
Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins.,Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. PMID:30046034[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S. Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. PMID:30046034 doi:http://dx.doi.org/10.1038/s41467-018-05092-w
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