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6f0s

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Crystal structure of Pizza6-SYW

Structural highlights

6f0s is a 1 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.5Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

beta-propeller proteins are highly symmetrical, being composed of a repeated motif with four anti-parallel beta-sheets arranged around a central axis. Recently we designed the first completely symmetrical beta-propeller protein, Pizza6, consisting of six identical tandem repeats. Pizza6 is expected to prove a useful building block for bionanotechnology, and also a tool to investigate the folding and evolution of beta-propeller proteins. Folding studies are made difficult by the high stability and the lack of buried Trp residues to act as monitor fluorophores, so we have designed and characterized several Trp-containing Pizza6 derivatives. In total four proteins were designed, of which three could be purified and characterized. Crystal structures confirm these mutant proteins maintain the expected structure, and a clear redshift of Trp fluorescence emission could be observed upon denaturation. Among the derivative proteins, Pizza6-AYW appears to be the most suitable model protein for future folding/unfolding kinetics studies as it has a comparable stability as natural beta-propeller proteins.

Design of tryptophan-containing mutants of the symmetrical Pizza protein for biophysical studies.,Noguchi H, Mylemans B, De Zitter E, Van Meervelt L, Tame JRH, Voet A Biochem Biophys Res Commun. 2018 Mar 18;497(4):1038-1042. doi:, 10.1016/j.bbrc.2018.02.168. Epub 2018 Feb 23. PMID:29481797^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Noguchi H, Mylemans B, De Zitter E, Van Meervelt L, Tame JRH, Voet A. Design of tryptophan-containing mutants of the symmetrical Pizza protein for biophysical studies. Biochem Biophys Res Commun. 2018 Mar 18;497(4):1038-1042. doi:, 10.1016/j.bbrc.2018.02.168. Epub 2018 Feb 23. PMID:29481797 doi:http://dx.doi.org/10.1016/j.bbrc.2018.02.168

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6f0s"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Pizza6-SYW==
-<StructureSection load='6f0s' size='340' side='right' caption='[[6f0s]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+<StructureSection load='6f0s' size='340' side='right'caption='[[6f0s]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6f0s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F0S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F0S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6f0s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F0S FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f0s OCA], [http://pdbe.org/6f0s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f0s RCSB], [http://www.ebi.ac.uk/pdbsum/6f0s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f0s ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f0s OCA], [https://pdbe.org/6f0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f0s RCSB], [https://www.ebi.ac.uk/pdbsum/6f0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f0s ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 19: / Line 20: @@
 __TOC__
 </StructureSection>
-[[Category: Meervelt, L Van]]
+[[Category: Large Structures]]
-[[Category: Noguchi, H]]
+[[Category: Synthetic construct]]
-[[Category: Voet, A R.D]]
+[[Category: De Zitter E]]
-[[Category: Zitter, E De]]
+[[Category: Noguchi H]]
-[[Category: Artificial protein]]
+[[Category: Van Meervelt L]]
-[[Category: Beta propeller]]
+[[Category: Voet ARD]]
-[[Category: De novo protein]]

6f0s

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Current revision

Crystal structure of Pizza6-SYW

Structural highlights

Publication Abstract from PubMed

References

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