6frw
From Proteopedia
(Difference between revisions)
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==X-ray structure of the levansucrase from Erwinia tasmaniensis== | ==X-ray structure of the levansucrase from Erwinia tasmaniensis== | ||
| - | <StructureSection load='6frw' size='340' side='right'caption='[[6frw]]' scene=''> | + | <StructureSection load='6frw' size='340' side='right'caption='[[6frw]], [[Resolution|resolution]] 1.52Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FRW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6frw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Erwinia_tasmaniensis Erwinia tasmaniensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FRW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6frw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6frw OCA], [https://pdbe.org/6frw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6frw RCSB], [https://www.ebi.ac.uk/pdbsum/6frw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6frw ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.52Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6frw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6frw OCA], [https://pdbe.org/6frw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6frw RCSB], [https://www.ebi.ac.uk/pdbsum/6frw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6frw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/B2VCC3_ERWT9 B2VCC3_ERWT9] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Erwinia tasmaniensis is an epiphytic bacterium related to the plant pathogen Erwinia amylovora, the etiological agent of fire blight. In this study the levansucrase from E. tasmaniensis (EtLsc) has been compared with the homologous enzyme from E. amylovora (EaLsc). We characterized the enzymatic activity and compared the products profile of both enzymes by High Performance Anion Exchange Chromatography coupled with Pulsed Amperometric Detector (HPAEC-PAD). Moreover we determined the crystal structure of EtLsc to understand the structural peculiarity causing the different product profiles of the two homologues. EtLsc exhibits increased efficiency in the production of FOS, resulting in a better catalyst for biotechnological synthesis than EaLsc. Based on our results, we propose that the role of this enzyme in the life cycle of the two bacteria is most likely related to survival, rather than linked to pathogenicity in E. amylovora. | ||
| + | |||
| + | Comparison of the Levansucrase from the epiphyte Erwinia tasmaniensis vs its homologue from the phytopathogen Erwinia amylovora.,Polsinelli I, Caliandro R, Salomone-Stagni M, Demitri N, Rejzek M, Field RA, Benini S Int J Biol Macromol. 2019 Apr 15;127:496-501. doi:, 10.1016/j.ijbiomac.2019.01.074. Epub 2019 Jan 17. PMID:30660564<ref>PMID:30660564</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6frw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Erwinia tasmaniensis]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Benini S]] | [[Category: Benini S]] | ||
Current revision
X-ray structure of the levansucrase from Erwinia tasmaniensis
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