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| | <StructureSection load='6fuv' size='340' side='right'caption='[[6fuv]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='6fuv' size='340' side='right'caption='[[6fuv]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6fuv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bifidibacterium"_prevot_1938 "bifidibacterium" prevot 1938]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FUV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fuv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium Bifidobacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FUV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.001Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Blac_00785 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1678 "Bifidibacterium" Prevot 1938])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fuv OCA], [http://pdbe.org/6fuv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fuv RCSB], [http://www.ebi.ac.uk/pdbsum/6fuv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fuv ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fuv OCA], [https://pdbe.org/6fuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fuv RCSB], [https://www.ebi.ac.uk/pdbsum/6fuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fuv ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A4V8GZV8_9BIFI A0A4V8GZV8_9BIFI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bifidibacterium prevot 1938]] | + | [[Category: Bifidobacterium]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ejby, M]] | + | [[Category: Abou Hachem M]] |
| - | [[Category: Guskov, A]] | + | [[Category: Ejby M]] |
| - | [[Category: Hachem, M Abou]] | + | [[Category: Guskov A]] |
| - | [[Category: Slotboom, D J]] | + | [[Category: Slotboom DJ]] |
| - | [[Category: Abc-transporter]]
| + | |
| - | [[Category: Beta-mannotriose]]
| + | |
| - | [[Category: Solute binding protein]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
A0A4V8GZV8_9BIFI
Publication Abstract from PubMed
Human gut bifidobacteria rely on ATP-binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide-specific solute binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize BlMnBP1 and BlMnBP2, the two SBPs associated to the beta-manno-oligosaccharide (MnOS) ABC transporter in Bifidobacterium animalis subsp. lactis. Despite similar overall specificity and preference to mannotriose (Kd approximately 80 nM), affinity of BlMnBP1 is up to 2570-fold higher for disaccharides than BlMnBP2. Structural analysis revealed a substitution of an asparagine that recognizes the mannosyl at position 2 in BlMnBP1, by a glycine in BlMnBP2, which affects substrate affinity. Both substitution-types occur in bifidobacterial SBPs, but BlMnBP1-like variants prevail in human-gut isolates. B. animalis subsp. lactis ATCC27673 showed growth on gluco- and galactomannans and was able to outcompete a mannan-degrading Bacteroides ovatus strain in co-cultures, attesting the efficiency of this ABC uptake system. By contrast, a strain that lacks this transporter failed to grow on mannan. This study highlights SBP diversification as a possible strategy to modulate oligosaccharide uptake preferences of bifidobacterial ABC-transporters during adaptation to specific ecological niches. Efficient metabolism of galactomannan by distinct bifidobacteria, merits evaluating this plant glycan as a potential prebiotic. This article is protected by copyright. All rights reserved.
Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on beta-mannan possess distinct manno-oligosaccharide binding profiles.,Ejby M, Guskov A, Pichler MJ, Zanten GC, Schoof E, Saburi W, Slotboom DJ, Abou Hachem M Mol Microbiol. 2019 Apr 4. doi: 10.1111/mmi.14257. PMID:30947380[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ejby M, Guskov A, Pichler MJ, Zanten GC, Schoof E, Saburi W, Slotboom DJ, Abou Hachem M. Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on beta-mannan possess distinct manno-oligosaccharide binding profiles. Mol Microbiol. 2019 Apr 4. doi: 10.1111/mmi.14257. PMID:30947380 doi:http://dx.doi.org/10.1111/mmi.14257
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