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| | <StructureSection load='6g3b' size='340' side='right'caption='[[6g3b]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6g3b' size='340' side='right'caption='[[6g3b]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6g3b]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G3B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6G3B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6g3b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G3B FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1wte|1wte]], [[1wtd|1wtd]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">alr0933 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g3b OCA], [https://pdbe.org/6g3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g3b RCSB], [https://www.ebi.ac.uk/pdbsum/6g3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g3b ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6g3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g3b OCA], [http://pdbe.org/6g3b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g3b RCSB], [http://www.ebi.ac.uk/pdbsum/6g3b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g3b ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8YYB7_NOSS1 Q8YYB7_NOSS1] |
| - | The ability of 223 Type II restriction endonucleases to hydrolyze RNA-DNA heteroduplex oligonucleotide substrates was assessed. Despite the significant topological and sequence asymmetry introduced when one strand of a DNA duplex is substituted by RNA we find that six restriction enzymes (AvaII, AvrII, BanI, HaeIII, HinfI and TaqI), exclusively of the Type IIP class that recognize palindromic or interrupted-palindromic DNA sequences, catalyze robust and specific cleavage of both RNA and DNA strands of such a substrate. Time-course analyses indicate that some endonucleases hydrolyze phosphodiester bonds in both strands simultaneously whereas others appear to catalyze sequential reactions in which either the DNA or RNA product accumulates more rapidly. Such strand-specific variation in cleavage susceptibility is both significant (up to orders of magnitude difference) and somewhat sequence dependent, notably in relation to the presence or absence of uracil residues in the RNA strand. Hybridization to DNA oligonucleotides that contain endonuclease recognition sites can be used to achieve targeted hydrolysis of extended RNA substrates produced by in vitro transcription. The ability to 'restrict' an RNA-DNA hybrid, albeit with a limited number of restriction endonucleases, provides a method whereby individual RNA molecules can be targeted for site-specific cleavage in vitro.
| + | |
| - | | + | |
| - | Sequence-specific cleavage of RNA by Type II restriction enzymes.,Murray IA, Stickel SK, Roberts RJ Nucleic Acids Res. 2010 Dec;38(22):8257-68. doi: 10.1093/nar/gkq702. Epub 2010, Aug 11. PMID:20702422<ref>PMID:20702422</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6g3b" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anabaena 7120]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bochtler, M]] | + | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] |
| - | [[Category: Czapinska, H]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Kisiala, M]] | + | [[Category: Bochtler M]] |
| - | [[Category: Kowalska, M]] | + | [[Category: Czapinska H]] |
| - | [[Category: A-dna]] | + | [[Category: Kisiala M]] |
| - | [[Category: Hydrolase]] | + | [[Category: Kowalska M]] |
| - | [[Category: Restriction endonuclease]]
| + | |
| - | [[Category: Rna/dna heteroduplex]]
| + | |
| - | [[Category: Rna/dna hybrid]]
| + | |
| - | [[Category: Scanning complex]]
| + | |
