8w12

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Current revision (12:50, 9 May 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8w12 is ON HOLD until Paper Publication
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==Cryo-EM structure of VP3-VP6 heterohexamer==
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<StructureSection load='8w12' size='340' side='right'caption='[[8w12]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8w12]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bluetongue_virus_(serotype_1_/_isolate_South_Africa) Bluetongue virus (serotype 1 / isolate South Africa)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8W12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8W12 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8w12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8w12 OCA], [https://pdbe.org/8w12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8w12 RCSB], [https://www.ebi.ac.uk/pdbsum/8w12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8w12 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q1AE73_9REOV Q1AE73_9REOV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscopy (cryo-EM), cellular cryoelectron tomography (cryo-ET), and structure-guided mutagenesis to investigate genome packaging and capsid assembly of bluetongue virus (BTV), a member of the Reoviridae family of dsRNA viruses. A total of eleven assembly states of BTV capsid were captured, with resolutions up to 2.8 A, with most visualized in the host cytoplasm. ATPase VP6 was found underneath the vertices of capsid shell protein VP3 as an RNA-harboring pentamer, facilitating RNA packaging. RNA packaging expands the VP3 shell, which then engages middle- and outer-layer proteins to generate infectious virions. These revealed "duality" characteristics of the BTV assembly mechanism reconcile previous contradictory co-assembly and core-filling models and provide insights into the mysterious RNA packaging and capsid assembly of Reoviridae members and beyond.
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Authors:
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RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells.,Xia X, Sung PY, Martynowycz MW, Gonen T, Roy P, Zhou ZH Cell. 2024 Apr 25;187(9):2236-2249.e17. doi: 10.1016/j.cell.2024.03.007. Epub , 2024 Apr 12. PMID:38614100<ref>PMID:38614100</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 8w12" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Gonen T]]
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[[Category: Martynowycz MW]]
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[[Category: Roy P]]
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[[Category: Sung PY]]
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[[Category: Xia X]]
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[[Category: Zhou ZH]]

Current revision

Cryo-EM structure of VP3-VP6 heterohexamer

PDB ID 8w12

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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