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| | <StructureSection load='1xa7' size='340' side='right'caption='[[1xa7]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='1xa7' size='340' side='right'caption='[[1xa7]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1xa7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XA7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1xa7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XA7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PNM:OPEN+FORM+-+PENICILLIN+G'>PNM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PNM:OPEN+FORM+-+PENICILLIN+G'>PNM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa7 OCA], [https://pdbe.org/1xa7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xa7 RCSB], [https://www.ebi.ac.uk/pdbsum/1xa7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xa7 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa7 OCA], [http://pdbe.org/1xa7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xa7 RCSB], [http://www.ebi.ac.uk/pdbsum/1xa7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xa7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BLAR_STAAU BLAR_STAAU]] BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase. | + | [https://www.uniprot.org/uniprot/BLAR_STAAU BLAR_STAAU] BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chambers, H F]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Hills, T L]] | + | [[Category: Chambers HF]] |
| - | [[Category: Strynadka, N C]] | + | [[Category: Hills TL]] |
| - | [[Category: Wilke, M S]] | + | [[Category: Strynadka NC]] |
| - | [[Category: Zhang, H Z]] | + | [[Category: Wilke MS]] |
| - | [[Category: Antibiotic resistance]] | + | [[Category: Zhang HZ]] |
| - | [[Category: Benzylpenicillin]]
| + | |
| - | [[Category: Beta-lactam]]
| + | |
| - | [[Category: Beta-lactamase]]
| + | |
| - | [[Category: Blar1]]
| + | |
| - | [[Category: Penicillin g]]
| + | |
| - | [[Category: Sensor domain]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
BLAR_STAAU BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Staphylococcus aureus is among the most prevalent and antibiotic-resistant of pathogenic bacteria. The resistance of S. aureus to prototypal beta-lactam antibiotics is conferred by two mechanisms: (i) secretion of hydrolytic beta-lactamase enzymes and (ii) production of beta-lactam-insensitive penicillin-binding proteins (PBP2a). Despite their distinct modes of resistance, expression of these proteins is controlled by similar regulation systems, including a repressor (BlaI/MecI) and a multidomain transmembrane receptor (BlaR1/MecR1). Resistance is triggered in response to a covalent binding event between a beta-lactam antibiotic and the extracellular sensor domain of BlaR1/MecR1 by transduction of the binding signal to an intracellular protease domain capable of repressor inactivation. This study describes the first crystal structures of the sensor domain of BlaR1 (BlaRS) from S. aureus in both the apo and penicillin-acylated forms. The structures show that the sensor domain resembles the beta-lactam-hydrolyzing class D beta-lactamases, but is rendered a penicillin-binding protein due to the formation of a very stable acyl-enzyme. Surprisingly, conformational changes upon penicillin binding were not observed in our structures, supporting the hypothesis that transduction of the antibiotic-binding signal into the cytosol is mediated by additional intramolecular interactions of the sensor domain with an adjacent extracellular loop in BlaR1.
Crystal structures of the Apo and penicillin-acylated forms of the BlaR1 beta-lactam sensor of Staphylococcus aureus.,Wilke MS, Hills TL, Zhang HZ, Chambers HF, Strynadka NC J Biol Chem. 2004 Nov 5;279(45):47278-87. Epub 2004 Aug 18. PMID:15322076[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wilke MS, Hills TL, Zhang HZ, Chambers HF, Strynadka NC. Crystal structures of the Apo and penicillin-acylated forms of the BlaR1 beta-lactam sensor of Staphylococcus aureus. J Biol Chem. 2004 Nov 5;279(45):47278-87. Epub 2004 Aug 18. PMID:15322076 doi:10.1074/jbc.M407054200
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