1s3k
From Proteopedia
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'''Crystal Structure of a Humanized Fab (hu3S193) in Complex with the Lewis Y Tetrasaccharide''' | '''Crystal Structure of a Humanized Fab (hu3S193) in Complex with the Lewis Y Tetrasaccharide''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S3K OCA]. | |
==Reference== | ==Reference== | ||
Structural convergence of antibody binding of carbohydrate determinants in Lewis Y tumor antigens., Ramsland PA, Farrugia W, Bradford TM, Mark Hogarth P, Scott AM, J Mol Biol. 2004 Jul 16;340(4):809-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15223322 15223322] | Structural convergence of antibody binding of carbohydrate determinants in Lewis Y tumor antigens., Ramsland PA, Farrugia W, Bradford TM, Mark Hogarth P, Scott AM, J Mol Biol. 2004 Jul 16;340(4):809-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15223322 15223322] | ||
| - | [[Category: Mus musculus and homo sapiens]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Bradford, T M.]] | [[Category: Bradford, T M.]] | ||
[[Category: Farrugia, W.]] | [[Category: Farrugia, W.]] | ||
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[[Category: Ramsland, P A.]] | [[Category: Ramsland, P A.]] | ||
[[Category: Scott, A M.]] | [[Category: Scott, A M.]] | ||
| - | [[Category: | + | [[Category: Antigen binding fragment]] |
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Humanized antibody]] |
| - | [[Category: | + | [[Category: Immunoglobulin fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:15:12 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 05:15, 3 May 2008
Crystal Structure of a Humanized Fab (hu3S193) in Complex with the Lewis Y Tetrasaccharide
Overview
Antibodies targeting human epithelial carcinomas bearing Lewis Y (Le(y)) carbohydrate antigens provide a striking illustration of convergent immune recognition. We report a 1.9A resolution crystal structure of the Fab of a humanized antibody (hu3S193) in complex with the Le(y) tetrasaccharide, Fuc(alpha 1-->2)Gal(beta 1-->4)[Fuc(alpha 1-->3)]GlcNAc. Comparisons of the hu3S193 and BR96 antibodies bound to Le(y) tumor antigens revealed extremely similar mechanisms for recognition of the carbohydrate epitopes. Solvent plays a critical role in hu3S193 antibody binding to the Le(y) carbohydrate epitope. Specificity for Le(y) is maintained because a conserved pocket accepts an N-acetyl group of the core Gal(beta 1-->4)GlcNAc disaccharide. Closely related blood-group determinants (Le(a) and Le(b)) cannot enter the specificity pocket, making the Le(y) antibodies promising candidates for immunotherapy of epithelial cancer.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural convergence of antibody binding of carbohydrate determinants in Lewis Y tumor antigens., Ramsland PA, Farrugia W, Bradford TM, Mark Hogarth P, Scott AM, J Mol Biol. 2004 Jul 16;340(4):809-18. PMID:15223322 Page seeded by OCA on Sat May 3 08:15:12 2008
