2mwt

From Proteopedia

(Difference between revisions)

Current revision

NMR structure of crotalicidin in DPC micelles

Structural highlights

2mwt is a 1 chain structure with sequence from Crotalus durissus terrificus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAMP_CRODU Potent antimicrobial peptide against Gram-negative (P.aeruginosa, K.pneumoniae, E.coli, A.baumannii) (PubMed:25100358, PubMed:26465972). Shows moderate activities against Gram-positive bacteria (E.faecalis, S.aureus, S.pyogenes) (PubMed:25100358, PubMed:26465972). Also shows antifungal activity against standard and clinical isolates of yeasts (MIC=10-40 uM) and dermatophytes (MIC=1-5 uM) (PubMed:27876749). Adopts an amphipathic alpha helical conformation, that may allow to partition into the target membrane (PubMed:26465972). Shows high toxicity towards human fibroblasts (micromolar ranges) (PubMed:26465972). Shows low hemolytic activities on human erythrocytes (PubMed:25100358, PubMed:26465972). Also shows toxicity (micromolar ranges) when tested on many leukemia cell lines (PubMed:26465972). In addition, when tested in vitro on the parasite Trypanosoma cruzi (responsible of the Chagas disease), is able to reduce the number of the three forms (epimastigote, trypomastigote and amastigote) by inducing cell death through necrosis (PubMed:29208061).^[1] ^[2] ^[3]

Publication Abstract from PubMed

In silico dissection of crotalicidin (Ctn), a cathelicidin from a South American pit viper, yielded fragments Ctn[1-14] and Ctn[15-34], which were tested to ascertain to what extent they reproduced the structure and activity of the parent peptide. NMR data showing Ctn to be alpha-helical at the N-terminus and unstructured at the C-terminus were matched by similar data from the fragments. The peptides were tested against Gram-positive and -negative bacteria and for toxicity against both tumor and healthy cells. Despite its amphipathic alpha-helical structure, Ctn[1-14] was totally inert toward bacteria or eukaryotic cells. In contrast, unstructured Ctn[15-34] replicated the activity of parent Ctn against Gram-negative bacteria and tumor cells while being significantly less toxic toward eukaryotic cells. This selectivity for bacteria and tumor cells, plus a stability to serum well above that of Ctn, portrays Ctn[15-34] as an appealing candidate for further development as an anti-infective or antitumor lead.

Structural Dissection of Crotalicidin, a Rattlesnake Venom Cathelicidin, Retrieves a Fragment with Antimicrobial and Antitumor Activity.,Falcao CB, Perez-Peinado C, de la Torre BG, Mayol X, Zamora-Carreras H, Jimenez MA, Radis-Baptista G, Andreu D J Med Chem. 2015 Nov 12;58(21):8553-63. doi: 10.1021/acs.jmedchem.5b01142. Epub, 2015 Oct 26. PMID:26465972^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Falcao CB, de La Torre BG, Pérez-Peinado C, Barron AE, Andreu D, Rádis-Baptista G. Vipericidins: a novel family of cathelicidin-related peptides from the venom gland of South American pit vipers. Amino Acids. 2014 Nov;46(11):2561-71. PMID:25100358 doi:10.1007/s00726-014-1801-4
↑ Falcao CB, Perez-Peinado C, de la Torre BG, Mayol X, Zamora-Carreras H, Jimenez MA, Radis-Baptista G, Andreu D. Structural Dissection of Crotalicidin, a Rattlesnake Venom Cathelicidin, Retrieves a Fragment with Antimicrobial and Antitumor Activity. J Med Chem. 2015 Nov 12;58(21):8553-63. doi: 10.1021/acs.jmedchem.5b01142. Epub, 2015 Oct 26. PMID:26465972 doi:http://dx.doi.org/10.1021/acs.jmedchem.5b01142
↑ Bandeira ICJ, Bandeira-Lima D, Mello CP, Pereira TP, De Menezes RRPPB, Sampaio TL, Falcão CB, Rádis-Baptista G, Martins AMC. Antichagasic effect of crotalicidin, a cathelicidin-like vipericidin, found in Crotalus durissus terrificus rattlesnake's venom gland. Parasitology. 2018 Jul;145(8):1059-1064. PMID:29208061 doi:10.1017/S0031182017001846
↑ Falcao CB, Perez-Peinado C, de la Torre BG, Mayol X, Zamora-Carreras H, Jimenez MA, Radis-Baptista G, Andreu D. Structural Dissection of Crotalicidin, a Rattlesnake Venom Cathelicidin, Retrieves a Fragment with Antimicrobial and Antitumor Activity. J Med Chem. 2015 Nov 12;58(21):8553-63. doi: 10.1021/acs.jmedchem.5b01142. Epub, 2015 Oct 26. PMID:26465972 doi:http://dx.doi.org/10.1021/acs.jmedchem.5b01142

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mwt"

Categories: Crotalus durissus terrificus | Large Structures | Jimenez M | Zamora-Carreras H

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2mwt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crotalus_durissus_terrificus Crotalus durissus terrificus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MWT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwt OCA], [https://pdbe.org/2mwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mwt RCSB], [https://www.ebi.ac.uk/pdbsum/2mwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwt ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwt OCA], [https://pdbe.org/2mwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mwt RCSB], [https://www.ebi.ac.uk/pdbsum/2mwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mwt ProSAT]</span></td></tr>
 </table>
 == Function ==

2mwt

From Proteopedia

Current revision

NMR structure of crotalicidin in DPC micelles

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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