1ycd

From Proteopedia

(Difference between revisions)

Revision as of 08:02, 15 May 2024

Crystal structure of yeast FSH1/YHR049W, a member of the serine hydrolase family

Structural highlights

1ycd is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FSH1_YEAST Serine hydrolase of unknown specificity.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Yhr049w/FSH1 was recently identified in a combined computational and experimental proteomics analysis for the detection of active serine hydrolases in yeast. This analysis suggested that FSH1 might be a serine-type hydrolase belonging to the broad functional alphabeta-hydrolase superfamily. In order to get insight into the molecular function of this gene, it was targeted in our yeast structural genomics project. The crystal structure of the protein confirms that it contains a Ser/His/Asp catalytic triad that is part of a minimal alpha/beta-hydrolase fold. The architecture of the putative active site and analogies with other protein structures suggest that FSH1 may be an esterase. This finding was further strengthened by the unexpected presence of a compound covalently bound to the catalytic serine in the active site. Apparently, the enzyme was trapped with a reactive compound during the purification process.

Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family.,Quevillon-Cheruel S, Leulliot N, Graille M, Hervouet N, Coste F, Benedetti H, Zelwer C, Janin J, Van Tilbeurgh H Protein Sci. 2005 May;14(5):1350-6. Epub 2005 Mar 31. PMID:15802654^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Baxter SM, Rosenblum JS, Knutson S, Nelson MR, Montimurro JS, Di Gennaro JA, Speir JA, Burbaum JJ, Fetrow JS. Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast. Mol Cell Proteomics. 2004 Mar;3(3):209-25. Epub 2003 Nov 25. PMID:14645503 doi:http://dx.doi.org/10.1074/mcp.M300082-MCP200
↑ Quevillon-Cheruel S, Leulliot N, Graille M, Hervouet N, Coste F, Benedetti H, Zelwer C, Janin J, Van Tilbeurgh H. Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family. Protein Sci. 2005 May;14(5):1350-6. Epub 2005 Mar 31. PMID:15802654 doi:10.1110/ps.051415905

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ycd"

@@ Line 3: / Line 3: @@
 <StructureSection load='1ycd' size='340' side='right'caption='[[1ycd]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ycd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YCD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ycd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YCD FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI5:2-HYDROXY-4,5-DIOXOHEPTYL+HYDROGEN+PHOSPHONATE'>LI5</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YHR049w ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI5:2-HYDROXY-4,5-DIOXOHEPTYL+HYDROGEN+PHOSPHONATE'>LI5</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ycd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ycd OCA], [https://pdbe.org/1ycd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ycd RCSB], [https://www.ebi.ac.uk/pdbsum/1ycd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ycd ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/FSH1_YEAST FSH1_YEAST]] Serine hydrolase of unknown specificity.<ref>PMID:14645503</ref>
+[https://www.uniprot.org/uniprot/FSH1_YEAST FSH1_YEAST] Serine hydrolase of unknown specificity.<ref>PMID:14645503</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
 [[Category: Large Structures]]
-[[Category: Coste, F]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Graille, M]]
+[[Category: Coste F]]
-[[Category: Janin, J]]
+[[Category: Graille M]]
-[[Category: Leulliot, N]]
+[[Category: Janin J]]
-[[Category: Quevillon-Cheruel, S]]
+[[Category: Leulliot N]]
-[[Category: Tilbeurgh, H van]]
+[[Category: Quevillon-Cheruel S]]
-[[Category: Structural genomic]]
+[[Category: Van Tilbeurgh H]]
-[[Category: Esterase]]
-[[Category: Lipase]]
-[[Category: S. cerevisiae]]
-[[Category: Serine hydrolase]]
-[[Category: Unknown function]]
-[[Category: Ysg]]

1ycd

From Proteopedia

Revision as of 08:02, 15 May 2024

Crystal structure of yeast FSH1/YHR049W, a member of the serine hydrolase family

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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