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| | ==Solution Structure of the pelle Death Domain== | | ==Solution Structure of the pelle Death Domain== |
| - | <StructureSection load='1ygo' size='340' side='right'caption='[[1ygo]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1ygo' size='340' side='right'caption='[[1ygo]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ygo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YGO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ygo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YGO FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pll ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Transferase Transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1, 2.7.11.8, 2.7.11.9, 2.7.11.10, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.21, 2.7.11.22, 2.7.11.24, 2.7.11.25, 2.7.11.30 and 2.7.12.1 2.7.11.1, 2.7.11.8, 2.7.11.9, 2.7.11.10, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.21, 2.7.11.22, 2.7.11.24, 2.7.11.25, 2.7.11.30 and 2.7.12.1] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ygo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygo OCA], [https://pdbe.org/1ygo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ygo RCSB], [https://www.ebi.ac.uk/pdbsum/1ygo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygo ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ygo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygo OCA], [https://pdbe.org/1ygo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ygo RCSB], [https://www.ebi.ac.uk/pdbsum/1ygo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/KPEL_DROME KPEL_DROME]] Plays an essential role in the Tl receptor signaling pathway that establishes embryonic dorsoventral polarity; the signal directs import of dl into ventral and ventrolateral nuclei, thereby establishing dorsoventral polarity. Tub recruits pll to the plasma membrane and protein-protein interaction activates pll.<ref>PMID:8440018</ref> <ref>PMID:7527496</ref> <ref>PMID:7635064</ref> <ref>PMID:10330490</ref>
| + | [https://www.uniprot.org/uniprot/KPEL_DROME KPEL_DROME] Plays an essential role in the Tl receptor signaling pathway that establishes embryonic dorsoventral polarity; the signal directs import of dl into ventral and ventrolateral nuclei, thereby establishing dorsoventral polarity. Tub recruits pll to the plasma membrane and protein-protein interaction activates pll.<ref>PMID:8440018</ref> <ref>PMID:7527496</ref> <ref>PMID:7635064</ref> <ref>PMID:10330490</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Transferase]]
| + | [[Category: Gay NJ]] |
| - | [[Category: Gay, N J]] | + | [[Category: Moncrieffe MC]] |
| - | [[Category: Moncrieffe, M C]] | + | [[Category: Stott KM]] |
| - | [[Category: Stott, K M]] | + | |
| - | [[Category: Anti-parallel 6 helix bundle]]
| + | |
| Structural highlights
Function
KPEL_DROME Plays an essential role in the Tl receptor signaling pathway that establishes embryonic dorsoventral polarity; the signal directs import of dl into ventral and ventrolateral nuclei, thereby establishing dorsoventral polarity. Tub recruits pll to the plasma membrane and protein-protein interaction activates pll.[1] [2] [3] [4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The interaction between the death domains (DDs) of Tube and the protein kinase Pelle is an important component of the Toll pathway. Published crystallographic data suggests that the Pelle-Tube DD interface is plastic and implies that in addition to the two predominant Pelle-Tube interfaces, a third interaction is possible. We present the NMR solution structure of the isolated death domain of Pelle and a study of the interaction between the DDs of Pelle and Tube. Our data suggests the solution structure of the isolated Pelle DD is similar to that of Pelle DD in complex with Tube. Additionally, they suggest that the plasticity observed in the crystal structure may not be relevant in the functioning death domain complex.
Solution structure of the isolated Pelle death domain.,Moncrieffe MC, Stott KM, Gay NJ FEBS Lett. 2005 Jul 18;579(18):3920-6. PMID:16004997[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shelton CA, Wasserman SA. pelle encodes a protein kinase required to establish dorsoventral polarity in the Drosophila embryo. Cell. 1993 Feb 26;72(4):515-25. PMID:8440018
- ↑ Grosshans J, Bergmann A, Haffter P, Nusslein-Volhard C. Activation of the kinase Pelle by Tube in the dorsoventral signal transduction pathway of Drosophila embryo. Nature. 1994 Dec 8;372(6506):563-6. PMID:7527496 doi:http://dx.doi.org/10.1038/372563a0
- ↑ Galindo RL, Edwards DN, Gillespie SK, Wasserman SA. Interaction of the pelle kinase with the membrane-associated protein tube is required for transduction of the dorsoventral signal in Drosophila embryos. Development. 1995 Jul;121(7):2209-18. PMID:7635064
- ↑ Grosshans J, Schnorrer F, Nusslein-Volhard C. Oligomerisation of Tube and Pelle leads to nuclear localisation of dorsal. Mech Dev. 1999 Mar;81(1-2):127-38. PMID:10330490
- ↑ Moncrieffe MC, Stott KM, Gay NJ. Solution structure of the isolated Pelle death domain. FEBS Lett. 2005 Jul 18;579(18):3920-6. PMID:16004997 doi:10.1016/j.febslet.2005.06.009
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