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| | <StructureSection load='1yv6' size='340' side='right'caption='[[1yv6]], [[Resolution|resolution]] 1.78Å' scene=''> | | <StructureSection load='1yv6' size='340' side='right'caption='[[1yv6]], [[Resolution|resolution]] 1.78Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1yv6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Litpi Litpi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YV6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1yv6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lithobates_pipiens Lithobates pipiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YV6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1yv4|1yv4]], [[1yv7|1yv7]]</div></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yv6 OCA], [https://pdbe.org/1yv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1yv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yv6 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yv6 OCA], [https://pdbe.org/1yv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1yv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yv6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/RNP30_RANPI RNP30_RANPI]] Basic protein with antiproliferative/cytotoxic activity against several tumor cell lines in vitro, as well as antitumor in vivo. It exhibits a ribonuclease-like activity against high molecular weight ribosomal RNA.
| + | [https://www.uniprot.org/uniprot/RNP30_LITPI RNP30_LITPI] Basic protein with antiproliferative/cytotoxic activity against several tumor cell lines in vitro, as well as antitumor in vivo. It exhibits a ribonuclease-like activity against high molecular weight ribosomal RNA. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Litpi]] | + | [[Category: Lithobates pipiens]] |
| - | [[Category: Carannante, A]] | + | [[Category: Carannante A]] |
| - | [[Category: Donato, A Di]] | + | [[Category: Di Donato A]] |
| - | [[Category: Fiore, A Di]] | + | [[Category: Di Fiore A]] |
| - | [[Category: Mazzarella, L]] | + | [[Category: Mazzarella L]] |
| - | [[Category: Merlino, A]] | + | [[Category: Merlino A]] |
| - | [[Category: Notomista, E]] | + | [[Category: Notomista E]] |
| - | [[Category: Sica, F]] | + | [[Category: Sica F]] |
| - | [[Category: Antitumor action]]
| + | |
| - | [[Category: Dynamic]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Onconase thermal stability]]
| + | |
| - | [[Category: Ribonuclease]]
| + | |
| - | [[Category: Small conformational change]]
| + | |
| Structural highlights
Function
RNP30_LITPI Basic protein with antiproliferative/cytotoxic activity against several tumor cell lines in vitro, as well as antitumor in vivo. It exhibits a ribonuclease-like activity against high molecular weight ribosomal RNA.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Onconase (ONC), a member of the RNase A superfamily extracted from oocytes of Rana pipiens, is an effective cancer killer. It is currently used in treatment of various forms of cancer. ONC antitumor properties depend on its ribonucleolytic activity that is low in comparison with other members of the superfamily. The most damaging side effect from Onconase treatment is renal toxicity, which seems to be caused by the unusual stability of the enzyme. Therefore, mutants with reduced thermal stability and/or increased catalytic activity may have significant implications for human cancer chemotherapy. In this context, we have determined the crystal structures of two Onconase mutants (M23L-ONC and C87S,des103-104-ONC) and performed molecular dynamic simulations of ONC and C87S,des103-104-ONC with the aim of explaining on structural grounds the modifications of the activity and thermal stability of the mutants. The results also provide the molecular bases to explain the lower catalytic activity of Onconase compared with RNase A and the unusually high thermal stability of the amphibian enzyme.
The importance of dynamic effects on the enzyme activity: X-ray structure and molecular dynamics of onconase mutants.,Merlino A, Mazzarella L, Carannante A, Di Fiore A, Di Donato A, Notomista E, Sica F J Biol Chem. 2005 May 6;280(18):17953-60. Epub 2005 Feb 22. PMID:15728177[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Merlino A, Mazzarella L, Carannante A, Di Fiore A, Di Donato A, Notomista E, Sica F. The importance of dynamic effects on the enzyme activity: X-ray structure and molecular dynamics of onconase mutants. J Biol Chem. 2005 May 6;280(18):17953-60. Epub 2005 Feb 22. PMID:15728177 doi:10.1074/jbc.M501339200
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